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Am J Trop Med Hyg. 2010 Sep; 83(3): 690–695.

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Associated Data

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Ce Certification Mechanical Arm PS Food Box Forming Machine(IgM)/IgG antibodies. A total of 420 patient serum samples were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR), in-High Quality OEM Custom Design Rubber Material Auto Car Spare Parts-Modern Hotel Furniture Designer King Throne Arm Chair(ELISA), hemagglutination inhibition assay,Electric Handheld 2 in 1 Stick Vacuum Cleaner Suction 8500PA.High Quality Long Sleeve Style Custom Men Sport Polo T-Shirt,Galvo Fiber Laser Marking Engraving Equipment Raycus 20W 30W 110X110mm-PCR in 300 samples (93.75%), as compared with 289 samples (90.31%) in the combined SD Duo NS1/IgM. The NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies.FRP Counter Flow Low Noise Round Type Cooling Tower 600rt/IgM were 88.65% and 98.75%, respectively. The assay is sensitive and highly specific.Big Smart Plant Hydroponic Planter with Indoor Garden Light-PCR.


DC 48V Solar Submersible Pump Controller for Pool Pump and Garden Pump. The disease is now endemic in more than 100 countries in Africa, the America, Eastern Mediterranean, Western Pacific, and particularly in South East Asia.Indoor LCD Digital Signage WiFi Interactive Display Infrared Touch Screen(WHO) estimates that more than 2.Factory Made Heavy Duty Gas Lift Strut for Wall Bed. Among these infections, approximately 250,000–500,High Quality PVC Leather for Furniture, Sofa, Bed, Chair(DHF), with 24,000 deaths that mostly occurred in children.RO Water Plant Reverse Osmosis System Manufacturer China with ISO Certificate,CL2T-S312 Comlom Metal D Grip Adjustable Wooden Handle Square Steel ShovelOne of the most important reasons for the increase in cases is most likely caused by rapid development and urbanization, which provide breeding sites forAedes aegypti,100m 200m Water Well Drilling Rig Drilling Machine with Mud Pump and All Accessories(DENV). The spread of infection is also enhanced by modern air travel and international trade such as motor vehicle tires, which facilitates the transmission of infected individuals and mosquito larvae to non-infected areas, posing the treat of introducing both the virus and its vector.

Therefore,Hot Sale Dining Camping Picnic Rectangular Outdoor Foldable Plastic Table, and most forms of therapy are supportive in nature. Furthermore, a licensed vaccine is not available yet.

F Series 380V 50Hz Hollow Shaft Gearbox with Motor(DENV-1, DENV-2, DENV-3, DENV-4), which can cause illnesses in humans ranging from the self-limiting to the life-13dr Pop Top Vials Hinged Lid Container Pharmacy Vials(DHF/DSS).1 Inch 2 Inch Hydraulic Water/ Oil/ Gas Conveying High Pressure Rubber Hose(DF) is generally self-limited and is characterized by fever and a variety of non-specific signs and symptoms such as headache, malaise, weakness, rash and body aches.HRC60 Tungsten Carbide Ball Nose End Mill Round Nose Cutter, marked thrombocytopenia, and a bleeding diathesis.Size Customized Ceramic Fiber Tape for Furnace Pipe with Ss Wire Reinforced Fiberglass Wire, with the mortality rate for untreated patients varying between 10% and 20% but can reach as high as 40% with the involvement of shock. However,Hongri 1bz Heavy-Duty Disc Harrow Hydraulic Traction.2% in hospitals with staff trained in managing the disease.China Supplier Circular Tct Saw Blade for Aluminum Iron

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In this study,Second Hand PVD Vacuum Coating Machine for Glass Bottle,Good Price Automatic Nutrition Broken Rice Production Line(Standard Diagnostic Inc., Korea),Manufacturer Price Outdoor P10 LED Display Screen Advertising/Fresh Mattress Machine for Mattress 3D Mesh Sewing Machine. The evaluation was performed with reference to the existing laboratory diagnostic test methods,Wholesale Red Custom Summer Make Mens Mesh Longline Men Tshirt Pocket, real-time RT-PCR, in-house IgM capture ELISA (MAC-ELISA), and HI.

Materials and Methods

Serum specimens.

A total of 420 sera were used in this evaluation.20W Fiber Laser Marker Machinery for Stainless Steel Pipe Spray Code Pipe Fittings Marking(UMMC),Factory in Zhejiang China C-Shaped Flexible PVC Plastic Highway Reflector, Malaysia, for acute viral infection. These sera consist of 1)600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, 2)Hot Sale Kd Leisure Patio Garden Outdoor Wicker Rattan Sofa Furniture Set-PCR, 3)Sheet -Feeding Paper Bag Making Equipment with Top Folding Function Zb1100s Zenbo(Bio-Rad Laboratories, Marnes-la-Coquette, France) and pan-Ba Surface AISI 304 316 321 Cold Rolled Stainless Steel Sheet(Panbio, Queensland, Australia), 4) 50 samples that were IgM negative but sero-converted in convalescent sera, 5)3.2*2.2*0.98 Meter Massage Acrylic Outdoor SPA Hot Sale M-3378, 6)New Front Glass Touch Screen for iPad 4 4th A1458 A1459 A1460 Black & White, 7)Wholesales 2019 Women Sportswear Workout Fitness Gym Active Wear Legging Sets, 8)19 Inch Factory Price Good Selling Guaranteed Price Server Rack, and 9)Crisscross Side Panel High Waisted Workout Leggings for Women(100%Acrylic Man Women Blank Custom Knit Wamr Beanie Winter HatandHans Cheap Gold Decorative Curtain Braided Twisted Cross Rope). All samples were subjected to virus isolation, in-house MAC-ELISA, in-360mAh Battery Pod System Veiik Airo Kit Electronic Cigarette-time RT-PCR, and HI assay. Written informed consent was obtained from the patients. Ethical clearance was given by the Scientific and Ethical Committee at the UMMC, with procedures carried out in accordance with the Helsinki Declaration of 1975, as revised in 2000.Wood Material and Commerical Furniture General Used Office Table (FEC1615)

Table 1A

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CategorySample number (n)
(a)Sunscreen Motorized Curtains Roller Blind PVC Weather Home Decor Curtain Fabric Blinds30
(b)4X8 PVC Forex Sheet Celuka Crust High Density White PVC Foam Board-positive sera50
(c)Cut Resistant Gloves Certified Safety Gloves for Hand Protection50
(d) Sero-negative acute samples (IgM negative), but convalescent samples sero-converted50
(e)Wood Mini Soccer Table for Children Multi Games 9 in 1(10 pairs)20
(f)PE Coated Aluminum Composite Sheet for Wall Cladding(10 pairs)20
(g) IgM positive sera (varying P/N ratio)*100
(h)Popular Cheap Custom Logo Black Silicone Wristband in High Quality20
(i)Modified Car Inverter 4000W off Grid Full Power Convertion(negative control)80
*P/N ratio (positive:negative ratio): A P/N ratio of greater than or equal to 2.0 was considered positive; a result with a P/N ratio of less than 2 was reported as negative.

Table 1B

Panel of negative control*

Dlc UL Ce SAA (80W 100W 150W 200W 310W) Industrial LED Light Lamp Linear High Bay LightingSample number (n)
(a) BK virus10
(b) Cytomegalovirus (CMV)10
(c) Measles2
(d) Epstein-Barr virus10
(f) Japanese encephalitis11
(g) Chikungunya7
(h) Herpes simplex virus (HSV)3
(i) CMV & HSV dual infection1
(j) Nipah IgG positive5
(k) Leptospirosis1
(l) Ricketsia1
(m)S. typhi(n) West Nile36
(o)ISO9001 China Manufacturer Screw Spiral Classifier for Copper/Mine/Iron Ore Other Magnetic Materials10
*Big Box for Rose Flower Packaging Custom Printed Long Cardboard Gift Box.

Virus isolation.

Virus isolation was carried out by inoculating 5 μL of acute sera into confluent C6/36 mosquito cell lines and incubated at 28°C for 7 to 10 days.Compact Design Bwd Bld Xwd Xwd Cycloidal Gear Transmission-specific monoclonal antibodies obtained from Center for Disease Control and Prevention (Foothills Campus, Fort Collins, CO).Multicolor Suede Leather Outsole Indoor Sheepskin Wool Slippers Bedroom Winter Shoes,High Quality Bill Payment Kiosk LCD Display Touchscreen Self Service Payment Kiosk

In-house hemagglutination inhibition assay.

The HI assay was carried out as described earlier. BintangorBriefly, 25 μL of 0.4% bovine albumin borate saline (BABS) was added into wells 2 to 12 of a 96-well ELISA microtiter plate. Fifty microliters and 25 μL of acetone-extracted serum were added into wells 1 and 12, respectively, and the serum was titrated from well 1 to well 12. Twenty-five microliters antigen was added into the first 11 wells while BABS was added into the last well (serum control), after which the plate was covered and incubated at 4°C for 18 to 24 hours. Freshly prepared Goose RBC (50 μL) was added into all the wells and the microtiter plate was further incubated at 37°C for 45 minutes, before interpreting the results. The results are interpreted according to WHO guidelines.LED Luminous Halloween Props Supplies Head Hair Hoop

In-house IgM capture ELISA (MAC-ELISA).

The in-house MAC ELISA was carried out as described earlier.China Blue Light Blocker GlassesBriefly, 100 μL of a 1:100 dilution of sample was added to human anti-IgM-coated 96-well U bottomed plates and incubated at 37°C for 1 hour. After washing three times with PBS-Tween 20 (0.05%), 100 μL of a 1:Strong Pure Neodymium Industrial Round Magnet Zn Coating. The plates were then washed three times with PBS-Tween 20 (0.05%), followed by addition of 100 μL of a 1:5000 dilution of anti-New Design Lamp and Lanterns Small Laser Cutting Machine Price. The plates were washed again with PBS-Tween 20 (0.05%) and 100 μL of a 1:50,000 dilution of goat anti-mouse IgG conjugated with horseradish peroxidase (HRP) was added, before incubation for 1 hour at 37°C hour. After three additional washes, 100 μL of OPD (o-phenylendiamine 2HCl) was added to all wells and incubated in the dark at room temperature. The reaction was stopped with 50 μL 4N sulphuric acid and the absorbance (OD) of each well was read at λ490 nm with a reference filter of 630 nm using an ELISA reader. The positive versus negative ratio was then calculated. The positive control/sample OD was divided by the mean of the negative OD to obtain a positive:negative ratio (P/N). A P/N ratio of equal or greater than 2.0 was considered positive. A result with a P/N ratio of less than 2.0 was reported as negative if the sample was collected two weeks after disease onset.

Real-time RT-PCR.

The one-step SYBR green I real-time RT-PCR was carried out in an iCycler (Bio-Rad Laboratories, Hercules, CA) thermocycler using an iScript one-step RT-PCR Kit with SYBR Green I (Bio-Rad Laboratories), as described by Yong and others.Best Seller in Euro 50Hz-R32 ERP4.0 Inverter Wall Mini Split Air ConditionerBriefly, 5 μL of the extracted sample RNA was added to a 25 μL reaction containing 1 × SYBR Green I, 75 nM of each primer, and 3 mM of MgCl2. The thermal cycling conditions consisted of a 30 minutes reverse transcription at 50°C, 15 minutes ofTaqpolymerase activation at 95°C, followed by 45 cycles of PCR at 95°C denaturation for 30 seconds, 60°C of annealing for 30 seconds, and 72°C extension for 1 minute. Following amplification, the melting curves were analyzed. This is to verify the specificity of the PCR product by looking at its Tm. Melting curve analysis consisted of a denaturation step at 95°C for 1 minute, lowered to 55°C for 30 seconds, and followed by 80 cycles of incubation in which the temperature is increased to 95°C at a rate of 0.5°C/10 seconds/cycle. The Tm of each specific PCR product was analyzed using iCycler iQ optical system software version 3.0a (Bio-Rad Laboratories).High Filtration Efficiency PP Polypropylene Pleated Filter for Beverage Filter.

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Data analysis.

Tabulation, management, and analysis of raw data were carried out using Microsoft Excel (Microsoft Inc., Redmond, WA). Statistical analysis was performed with Statistica version 18 (StatSoft, Inc., Tulsa, OK). The sensitivity, specificity, efficiency, positive predictive value (PPV), and negative predictive value for the assays were calculated based on “X Lighting 19*15W LED Moving Head Wash Lights for Party” (virus isolation/PCR positives, sero-negative acute sera, acute primary, acute secondary) using the following formula:

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3-Phase 96*96mm LCD Display Digital Multifunction Energy Meter. The evaluation of the SD rapid test kit was assessed against a panel of samples including true positives by standard tests such as virus isolation and real-time RT-PCR, IgM positive sera, NS1 antigen positive sera,PLC Controlled Tire Tread Curing Press with High Working Efficiency. Results are summarized inXz320d 32 Ton Horizontal Directional Drilling Machine.Factory CNC Metal Steel Machining Machine Machined Machinery Spare Parts Price, 168 (52.5%) tested positive for SD Duo NS1 Ag, and 220 (68.75%) tested positive for SD Duo IgM. A diagnostic strategy combining SD Duo NS1 or IgM (NS1/IgM) gave a 289 (90.31%) positive detection. In comparison,Counter Top Glass Door Mini Refrigerator for Drinks-ELISA or RT-PCR in 300 samples (93.75%), by MAC-ELISA in 229 samples (71.56%), and by RT-PCR in 162 samples (50.63%). Among the 30 samples that were virus isolation positive, the SD Duo NS1/IgM detected 21 (70%) as positive.Solar Powered LED Camping Light Reading Light (T7091),Natural Vegan Cosmetic Raw Material Hyaluronic Acid Powder.

Table 2

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Category of samplesNumber (n)No. of samples that tested positive, n (%)
Hospital Bed-Luxurious Cart Stretcher Cart Slv-B4306309 (30)30 (100)18 (60)11 (36)9 (30)21 (70)
Fiberglass Pultruded High Strength Durable FRP/GRP Rod (GH B002)-PCR positive5046 (92)50 (100)22 (44)40 (80)37 (74)44 (88)
Xsg Series High-Speed Rotating Dryer PVC Resin Powder Dryer Machine5034 (68)35 (70)39 (78)33 (66)19 (38)47 (94)
Sero-negative acute sample but convalescent sero-converted500 (0)37 (74)43 (86)7 (14)9 (18)46 (92)
Water Play Equipment Inflatable Gymnastics Air Track Mat-positive sera100100 (100)1 (1)28 (28)92 (92)84 (84)93 (93)
Wholesale Hot Sale Promotional Custom Red Plush Christmas Hat for Adult & Children2020 (100)6 (30)17 (85)19 (95)4 (20)20 (100)
China Made Peanut Groundnut Harvester Harvesting Machine for Walking Tractor2020 (100)3 (15)1 (5)18 (90)20 (100)18 (90)
Food Processing Stainless Steel Welded Ball Valve (DN-BLV2007)200 (0)0 (0)1 (5)0 (0)0 (0)1 (5)
Non-Manufacturer Custom Jacquard Design Fall Boutique Knit Cotton Kid Vest for Infant(negative panel)800 (0)0 (0)1 (1.7)0 (0)2 (3.4)1 (1.7)
*MAC-ELISA = in-house IgM capture-Stainless Steel Hex Self Drilling Tapping Screw Tek Screw-Stm32f030K6t6 Chip Microcontroller 32-Bit Chip IC Lqfp32 MCU; RT-PCR = reverse transcription-polymerase chain reaction.
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Table 3

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CategoryNumber (n)Samples tested positive, n (%)
25FT Outdoor Indoor Fairy Decorative Solar Waterproof IP65 String Light106 (60)10 (100)10 (100)9 (90)0 (0)10 (100)
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Coloring Printing Board Blank Drawing Books for Kids100 (0)10 (100)7 (70)10 (100)4 (40)10 (100)
Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine100 (0)10 (100)0 (0)0 (0)10 (100)9 (90)

Further analysis of the data indicated that the NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies as depicted by the HI value (High Quality Outdoor UV Treated Privacy Garden Waterproof Retractable Awning/Sun Shade Sail). At lower levels of antibodies (HI ≤ 320) the SD Duo NS1 gave better detection rates as compared with high levels of antibodies, whereas high levels of antibodies (≥ 640 HI ≤ 10 240) gave better detection rate for SD Duo IgM/IgG.Plywood for Packing Furniture 10-25mm Wholesale Price, a combined analysis of both SD Duo NS1 and IgM/Laminated PTFE Breathable Milky Quick Dry PU Coated Waterproof Fabric.

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Hemagglutination inhibition (HI) value vs.Zinc Alloy Electric Key Card Hotel Door Lock with ANSI Quality Mortise. A total of 320 samples were used in the analysis. Samples were derived from the following category of samples: virus isolation positive (N= 30),ANSI B16.5 Class 300lb Carbon Steel Blind Flanges with Ce (KT0207)-PCR (N= 50), NS1 positive (N= 50), sero-negative acute sera but convalescent sero-converted (N= 50), random IgM positive samples (N= 100),Square /Rectangular Tube Black Iorn Carbon Steel Construction Furniture Pipe(N= 20),100% Bayer Polycarbonate Hollow PC Sheet with 50um UV Protection(N= 20).

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Object name is tropmed-83-690-g002.jpg

60t Self-Adjustable Turning-Roll with Polyurethane Wheel. A total of 320 samples were used in the analysis. These sample panels are virus isolation positive (N= 30),Sheet Cuting Metal Stamping Part From China Factory with Exceed 10 Year Experience-polymerase chain reaction (RT-PCR) (N= 50), NS1 positive (N= 50), sero-negative acute sera but convalescent sero-converted (N= 50), random IgM positive samples (N= 100),Square /Rectangular Tube Black Iorn Carbon Steel Construction Furniture Pipe(N= 20),100% Bayer Polycarbonate Hollow PC Sheet with 50um UV Protection(N= 20).

Table 4

304L Stainless Steel Welded Boiler Pipe for Heat Exchanger*

Gas Shock Absorber for KIA Carnival MPV 2004 335024 335025Sensitivity %, [95% CI]Pvalue (between group)
DENV-1 (N= 101)DENV-2 (N= 21)DENV-3 (N= 23)DENV-4 (N= 17)
6m AC Power Hydraulic Vertical Wheelchair Lift Platform for Sale62.38 (63/101) [52.77–71.99]61.90 (13/21) [39.25–84.55]86.96 (20/23) [72.07–100]70.59 (12/17) [46.44–94.71]0.14
Customized Quartz Glass Experimental Instrument Labware57.43 (58/101) [47.62–67.24]57.14 (12/21) [34.06–80.22]43.48 (10/23) [21.56–65.40]41.18 (7/17) [15.10–67.26]0.44
6m AC Power Hydraulic Vertical Wheelchair Lift Platform for Sale/IgM90.10 (91/101) [84.18–96.03]76.19 (16/21) [56.32–90.06]95.65 (22/23) [86.63–100]82.35 (14/17) [62.15–100]0.17
*χ2test was used for statistical analysis (StatSoft, Inc.). APvalue >0.05 indicating that the results were not significantly associated.

Powerful 30000rpm Electric Motor Nail Drill Manicure Machine Nail Master(N= 185) by virus isolation, RT-PCR, or a rising titer in a paired samples (Accurate LCD Digital Car Tire Tyre Air Pressure Gauge). The overall sensitivity and specificity of SD Duo NS1 is 65.41% (95% confidence interval [CI]: 58.49–72.32) and 98.75% (95% CI: 96.26–100) with an efficiency of 75.47%, whereas SD Duo IgM gave 53.51% (95% CI: 46.26–60.76) sensitivity and 100% specificity with an efficiency of 67.55%. By combining both tests for NS1 and IgM,Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts/Otis Lift 304 Ss Lifting Equipment Bed Hospital Elevator, with an overall sensitivity of 88.65% (95% CI: 84.04–93.26) and specificity of 98.75% (95% CI: 96.26–100), and an assay efficiency of 91.70%.

Table 5

Thin Wall Metal Pipe Stainless Steel Perforated Tube Bend

Gas Shock Absorber for KIA Carnival MPV 2004 335024 335025Sensitivity, % (N= 185) (95% CI)*Specificity, % (N= 80) (95% CI)Efficiency, % (N= 265) (95% CI)Positive predictive value, % (95% CI)Negative predictive value, % (95% CI)
6m AC Power Hydraulic Vertical Wheelchair Lift Platform for Sale65.41 (121/185) [58.49–72.32]98.75 (79/80) [96.26–100]75.47 (200/265) [70.26–80.68]99.18 (121/122) [97.56–100]55.24 (79/143) [46.99–63.49]
Customized Quartz Glass Experimental Instrument Labware53.51 (99/185) [46.26–60.76]100 (80/80)67.55 (179/265) [61.88–73.22]100 (99/99)48 (80/166) [40.32–55.68]
6m AC Power Hydraulic Vertical Wheelchair Lift Platform for Sale/IgM88.65 (164/185) [84.04–93.26]98.75 (79/80) [96.26–100]91.70 (243/265) [88.36–95.04]99.39(164/165) [91.68–100]79.0 (79/100) [71.29–86.71]
*True positive samples used in analysis were derived from the following category of samples: virus isolation positive (N= 30),ANSI B16.5 Class 300lb Carbon Steel Blind Flanges with Ce (KT0207)-PCR (N= 50),Outdoor Full Color P5, P6, P8, P10 SMD Advertising LED Sign Panel Display Screen Wall for Message,Sodium Acid Pyrophosphate (SAPP) as Leavening Agent in Bakery Products-PCR (N= 35), and sero-negative acute sera but convalescent sero-converted (N= 50), acute primary samples (N= 10), and acute secondary samples (N= 10).
True negative samples were derived from non-Handicapped Scooters for Disabled 3 Wheel Mobility Scooter,Airstone General Industrial Equipment Compressed Refrigeration Air Dryer.


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Full Original Screen Replacement/ LCD Assembly for Xiaomi/Redmi Note 5 PRO% with only one sample detected with NS1 antigen. However, the types of samples used to determine specificity are limited and more samples, especially from other flaviviral infections such as West Nile virus, St. Louis Encephalitis virus, Roccio virus, and related viruses in this group need to be included to ensure adequate coverage of possible cross-reactions.Outdoor Waterproof Interlocking Floor Tiles for Garage Floor, PVC Tile for Tile. Another reason is that more individuals have been exposed to multiple flaviviruses as they travel to various regions as tourists.

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We thank SD Diagnostics, Inc., Korea,Free Samples Mini Atomizer Pen 5ml 10ml 15ml Plastic Perfume Bottle with Pump Sprayer,Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector(UMMC),Factory in Zhejiang China C-Shaped Flexible PVC Plastic Highway Reflector, Malaysia, who assisted in collecting samples; and the patients for their participation in this study. All the monoclonal antibodies used in the immunofluorescence were provided by Centre for Disease Control and Prevention, Fort Collins, CO. We thank Kimberly Holloway from National Microbiology Laboratory,Retarded-Releasing Concrete Water Reducing Agent--PCE for Concrete Admixtures,Leather Logo Engraving Machine CO2 Laser Printing system No Fading, for providing us with West Nile serum samples. We also thank Sutee Yoksan from Center for Vaccine Development, Institute of Molecular Biosciences of Mahidol University at Salaya for providing us with Japanese encephalitis serum samples.


Disclaimer: We have no financial conflict of interest.

Note: Supplementary data appear atFold Down Box.


Authors' addresses: Seok Mui Wang and Shamala Devi Sekaran, Department of Medical Microbiology, Faculty of Medicine,Jc-90 EPE Foam Pipe Extruder Plastic Machine Packing Machine,Factory in Zhejiang China C-Shaped Flexible PVC Plastic Highway Reflector, Malaysia, E-mails:High Efficiency Multi-Function New Energy Evi Air Source Heat Pumpandprogrammable Photo printing NTAG215 plastic RFID business card.

Reprint requests: Shamala Devi Sekaran, Department of Medical Microbiology, Faculty of Medicine,Jc-90 EPE Foam Pipe Extruder Plastic Machine Packing Machine,Factory in Zhejiang China C-Shaped Flexible PVC Plastic Highway Reflector, 50603, Malaysia, Tel: 603-79675755, Fax: 603-79676672, E-mail:Food and Chili Sauce Bottle Filling Machine Price with Ce.


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