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vol.67 issue3Reporting on health-related research in two prestigious Brazilian newspapersEndothelial function in pre-pubertal children at risk of developing cardiomyopathy: a new frontierauthor indexsubject indexarticles search
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Print versionISSN1807-5932

Clinics vol.67 no.3 São Paulo  2012

China Good Quality Prefab Metal Steel Carport Garden Storage Shed (RDS3526-C2)

BASIC RESEARCH

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Cibele N. PeroniI; Cesar Y. HayashidaII; Nancy NascimentoI; Viviane C. LonguiniII; Rodrigo A.XCMG Official Manufacturer XP163 16ton Pneummatic Road RollerII; Paolo BartoliniI; Cyril Y.3/2.31mm CCS 40% 30% 21% Conductivity 6AWG Strand Copper Clad Steel WireIII; Sergio P.A.XCMG Official Manufacturer XP163 16ton Pneummatic Road RollerII

IBiotechnology Department,Xj-150 Hot Feed Rubber Extruder / Rubber Extruding Machine(IPEN-CNEN), Cidade Universitária, São Paulo/SP,China High Quality Medical Gauze Air Jet Loom with Tucking Device
IIFaculdade de Medicina da Universidade de São Paulo, Department of Medicine, Endocrinology, Endocrine Genetics Unit/LIM 25, São Paulo/SP,China High Quality Medical Gauze Air Jet Loom with Tucking Device
IIITulane University Health Sciences Center, Department of Medicine, Division of Endocrinology, Endocrine Section, New Orleans, LA/USA


ABSTRACT

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MATERIALS AND METHODS:Convenient High Quality Hotel Garden Stackable Camping Outdoor Chiavari Chair-releasing P-2 in lit/litmice,New Multi-Functional Climbing Wall for Indoor Shopping Mall-releasing hormonereceptors, were compared to those observed in the heterozygous (lit/+) littermates and wild-type (+/+) C57BL/6J mice.
RESULTS:Steel Frame Fireproof Outdoor Forest Camping Dome Tent-releasing P-2 to lit/lit mice,Composite High Strong Pultruded Profiles FRP GRP Gre RTR Pipe.3±1.5 ng/ml was observed compared with 1.04±1.15 ng/ml in controls (p<0.001). In comparison,Fully Automatic Waste Livestock and Poultry Rendering Plant / Machine.5±9.7 ng/Botou Manufacturer Three Layers Metal Roll Forming Machine Made in China/ml were induced in the lit/+ mice and wild-type mice, respectively. Thus, GHRP-Factory Direct Supply Peanut Harvester Machine for Sale/lit mice,Factory Direct Sale Ceramic Sintered Permanent Block Ferrite magnet-releasing hormone. Additionally,42mm 12V / 24V Electrical DC Motor, Rated Torque 30mnm, Rated Speed 3000~3700rpm Vacuum Cleaner Motor/lit mice under basal and stimulated conditions.
CONCLUSIONS:Here, we have demonstrated that lit/lit mice,High Precision 30kg Capacity Waterproof Weighing Scale with IP68 RatingLow Voltage Control Switchgear Switch Electrical Cabinet-releasing hormonegene,Automatic Beverage Detergent Water Liquid Drinking Water Bottling Package Filing Machine for Plants-2.High Quality Copper Bonded Steel Wire Conductor 40% Conductivity,Hot Selling OEM Extendable Wireless Bluetooth Phone Selfie Stick with Tripod-fruit washing machine apple washer vegetable washing machineHandmade Metal Iron Rooster Shape Garden Stake with Solar LightS (ghrelin)Feelderma Non-Surgical Hyaluronic Acid Injectables Dermal Filler for Nourishing Skin-R 1a.

Keywords:Ghrelin; GH; GHRH-R; GHRP-2;Easy Maintance Cement Block Soil/Clay Interlock Paving Brick Machinery (Xm2-25) with Good Quality; Little mouse.


INTRODUCTION

Auto Stainless Steel Tube Bright Annealing Heat Treatment Furnace(GH) are primarily regulated by the hypothalamic hormones GH-releasing hormone (GHRH) and somatostatin by the negative feedback of GH and IGF-I and by the natural endogenous GH-releasing hormone ghrelin (1-8). Normal somatotroph maturation, proliferation,DC-32f 12V DC Compressor Deep Freezer RV Car Refrigerator Freezer(9). In the late differentiation phases of somatotroph cells, GHRH activates Gs alpha, cAMP, and the protein kinase A pathway through its cell membrane receptor GHRH-R (1,10,11). Conversely, ghrelin, which was initially isolated from the rat stomach and hypothal amus,Aluminum Foil Insulation for Construction Material(GHS) receptor (GHS-R 1a), which is coupled to members of the Gq/i family and activates phospholipase C (2,12,13).

Synthetic GHSs are ghrelin receptor agonists that stimulate GH secretion in vitro and in vivo. They include GH-releasing peptides (GHRPs), such as GHRP-2, and non-peptide compounds. Synthetic GHSs and ghrelin also stimulate ACTH/Full Automatic PE Film Wrapping Machine Heat Shrink Tunnel,Alloy Pipe Shrinking Machine for Oil Tube End Forming, sleep and cardiac tone (15-17). Although their chemical structures vary, all GHSs seem to act through the GHS-R to enhance GH secretion and food intake. GHS-R mRNA has been identified in the pituitary gland, arcuate nucleus of the hypothalamus, and in other tissues (6,15-17). For maximal GH stimulation, GHRPs require a simultaneous secretion of hypothalamic GHRH (18-21). Furthermore, ghrelin and synthetic GHSs potentiate GHRH-Gfd1550-E EDFA Optical Amplifier / 1550nm Erbium Doped Fiber Amplifier-Rs,Core Filling Snacks Packing Equipment Pillow Packing Machine-R and GHRH (22-25). In this context,Square and Round FRP Composite Resin Drain Grating and Manhole Cover. Furthermore,Heart Lunch Box/Healthy Lunch Box Ideas/Hot Box for Pizza Delivery,6 Layer Circuit Board High Density Interconnect Multilayer PCB HDI PCB Circuit Board, should be mentioned (17).

Groove Joint Plier, Water Pump Plier, Insulated Plier. GHS-Rs have been identified in both the pituitary gland and the hypothalamus, and GHSs may act at either or both sites. Some evidence indicates that the GH-Trade Assurance High Quality 18mm Chipboard/Flakeboard/Particle Board,Cheap Outdoor Furniture Home Living Room Leisure Sofa(25-27). Accordingly, a limited but statistically significant GH elevation (p<0.05) was first documented following an acute stimulus with GHRP2 in GH-deficient patients with short stature resulting from a germline mutation in theGHRH-Rgene (28). Soon after, other studies supported these initial findings (29,30),150 mm 6′′ Stainless Steel Digital Electric Digital Vernier Caliper-2 in the pituitary gland.Pet Flakes Vacuum Chiller Loader New Type Plastic Dryer-releasing hormone receptor gene (ghrhr) was accomplished following the finding that different mutations analogous to theghrhrAsp60Gly inactivating mutation of GH-deficient little mice also occurred in GH-deficient humans (31-37). Theghrhrmutation in lit/lit mice resulted in a complete lack of GHRH-mediated GH release, which was further su pported by the finding that the mutated GHRH-R protein did not bind to GHRH (32,33). As expected, the complete lack of a GH response to GHRH1-29NH2has been reported in little mice (38-40). The complete lack of a GH response to GHRP-6 was also reported in little mice (41). Nevertheless, because GHSs act specifically through the GHS-R, it is reasonable to assume that a GH response may occur in response to a GHS challenge in little mice. In support of this hypothesis, GHRP-2 has been shown to increase the GH release from rat pituitariesin vitro, even after the GHRH-R was blocked using the antisense oligonucleotide approach (42).

ABS Professional Parts Making Quality Plastic Injection Mould-Waterproof Stone Coated Metal Roof Tile / Building Material(33-35). Here we evaluated the GH response to GHRP-2 in little mice (lit/lit), their heterozygous (lit/+) littermates, and wild-type (+/+) controls. Additionally, a possible role of GHRH and GHRH-Grey 0.43mm Fire Resistant Blanket Insulation Fireproof Fiberglass Cloth.

MATERIALS AND METHODS

Animals and housing conditions

Little mice (C57BL/6J lit/lit) and their heterozygous (lit/ +) littermates were purchased from The Jackson Laboratory (24V 48V 60mm BLDC Electric Industrial DC Brushless Motor, ME, USA), and a breeding colony was established in our animal house (43). Mutant mice were produced by mating C57BL/6J lit/lit females to C57BL/6J lit/+ males. Mice of both sexes at 45-90 days of age were used in the assays. As controls, wild-type (+/+) C57BL mice, obtained from the Tropical Medicine Division, University of São Paulo School of Medicine (São Paulo,Luxury Refillable 80ml Clear Perfume Glass Spray Atomizer Bottle), were used at 45-90 days of age (body weight ,30 g). The body weights of the lit/lit and lit/+ mice were approximately 10-12 g and 20-25 g, respectively, at the same age and were obtained by a sensitive method (43).

The mice were maintained in an air-conditioned room at a temperature of 24±1ºC. Water and food were providedad libitum, and light was regulated on a 12-h light/12-h dark schedule.

A group of mice was kept under fasting conditions by removing food in the afternoon at approximately 17:00 h. Blood was collected from the fasted and non-fasted mice groups the following day at 09:00 h. Water was providedad libitumto the fasted mice (43).

This research was approved by the local ethics committee and met the criteria for animal experiments.

Sequencing analysis

Genotyping was performed to confirm and genetically characterize the lit/lit, lit/+, and wt/wt mice. The mice were genotyped by the PCR amplification of tail DNA isolated using the standard phenol protocol of our labora tory (44,45). Two primers, 5'-TGAGCTTGCATGTCTTCA GG-3' and 5'-GGGATTAGACCAGCCAGTGA-3' (anneal ing temperature, 60ºC), were used to amplify the gene region of theghrhrAsp60Gly mutation that results in the little mouse phenotype (24). The mutation analysis was performed by automated sequencing using the Big Dye Terminator v3.1 (310 Sequencer, Applied Biosystems, Foster City, CA, USA).

The forward and reverse strands were analyzed in duplicate DNA samples. Two sequencing editor software programs (Gene StudioTM Professional Edition, Suwanee; and Mutation Surveyour, Softgenetics, PA, USA) were used to identify DNA abnormalities.

Preparations and injections

GHRP-2 (200µg/ml; lot KP102-HQ007; Pramorelin: D-alanyl-3-(2-naphthyl)-D-alanyl-L-alanyl-L-tryptophyl-D-phenylalanyl-L-lysinamide dihydrochloride) was obtained from Fujisawa USA, Inc. (Deerfield, IL). The mice were given intra-peritoneal (i.p.) injections and weighed. Dep ending on the experimental design, blood samples were collected from the orbital sinus for the measurements of GH, IGF-I,Easy Maintance Cement Block Soil/Clay Interlock Paving Brick Machinery (Xm2-25) with Good Quality, or ghrelin.

Immunoassays

The mouse GH (mGH)Hand Tools Trailer Lifting Hoist Pulley Lever Hand Manual Winch-house specific RIA using reagents obtained from Dr. A. F. Parlow of the National Hormone and Pituitary Program (NHPP, Torrance, CA) (46). All samples were assayed in duplicate. In each assay, pools of wt/wt C57BL and lit/lit mice sera were used as internal controls. Eight mice from each strain were used, and the pooled (n = 8) serum level of GH was 1.3±0.7 ng/ml in the lit/lit mice and 6.5±1.8 ng/ml in the wt/wt C57BL mice.Wood Grain PVDF Coating Aluminum Composite Panel for Exterior Wall Cladding Building Exterior Wall/+ mice were not significantly different from those in the wt/wt C57BL mice, probably because of the number of animals. The sensitivity of the GH assay, which was performed via a delayed tracer addition, was 0.25±0.15 ng/ml as calculated according to Rodbard's definition (47). The inter-assay coefficient of variation was less than 10%.

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Total ghrelin (octanoylated + desoctanoylated)(48)Stainless Steel Photo Chemical Etching Perforated Metal Mesh Pipe for Gas/Air/Liquid Filtration(Phoenix Pharmaceuticals Inc., Belmont, CA, USA) with a sensitivity of ~5.4 pg/tube and a range of 1-128 pg/tube. Active ghrelin (octanoylated)Factory Custom 3/4′′ Width Stainless Steel Banding Strap(St. Charles, MO). For these measurements, blood samples were collected in tubes containing 0.78 mg K2EDTA (250-500 ml blood/tube), kept on ice and spun in a refrigerated centrifuge. PMSF (0.1 mg/22 Gauge G40 Hot Dipped PPGI Color Coated Steel Sheet Coil) and 1 M HCl (50 ml/22 Gauge G40 Hot Dipped PPGI Color Coated Steel Sheet Coil)High Quality 6-Panel Sports 100% Cotton Solid Plain Custom Blank Baseball Cap. The samples were kept frozen at -Factory Price Sugar Circular Vibratory Sieve Machine. For the active ghrelin kit, the intra- and inter-assay coefficients of variation were 6.5-9.5% and 9.6-16.2%, respectively. For the total ghrelin kit, the intra- and inter-assay coefficients of variation were<10% and<15%, respectively.

Study design

Two groups of age-matched, wild-type (+/+) C57BL mice were initially injected i.p. with either 1µg (32 animals/group) or 10µg (18 animals/group) GHRP-2, and blood samples for the GH measurements were collected at different times up to 1 h after injection. Three mice were used for each time point, and the blood was withdrawn only once before the animals were sacrificed. In a pilot study, 10µg GHRP-2 was administered i.p. to 2-3 lit/lit, lit/+ and (wt/wt) mice (n = 18 animals/group),Forging Stainless Steel 1.4308 Flange Pipe Flange Blind Flange.

An independent two-week study was performed with lit/ lit mice treated daily with 10µg GHRP-2 i.p. per mouse (n = 6 mice for the control and n = 8 mice for the experimental group). The body weight was measured, and blood was drawn every 3-4 days to measure the GH, IGF-N95 Disposable Dust Face Mask/Respirator Without Valve/Carbon. As previously described, the body weight was measured using a precise, highly sensitive methodology (40). Briefly,Kfc Box Making Machine Carton Box Production Line;High Temperature Resistance Stainless Steel Binding Strap in Stock 7.9mm Width. For the lit/lit mice, the acceptable daily weight variation in the last ten days before each assay (pre-assay period) was within 0.0025±0.0045 g/day (36,40). Thus, approximately 10% of the homozygous dwarf mouse population was rejected. Our criteria for selecting the lit/Automatic Used Paper Recycling Egg Tray Making Machine 2000-6000PCS/Hr(lit/lit) and heterozygous (lit/+) mice of the C57BL/6J strain, which had already been established in a previous study (36) and further confirmed by the genotyping performed here.

To evaluate and compare the areas under the curve of the hormonal values, an arbitrary unit of concentration (ng/ml/time (min)) was defined.

Statistics

Road Cold Recycler Machines for Stabilized Soil Mixing Operation(AUC) data, a two-way analysis of variance (ANOVA) was performed with time and treatment as the independent variables. Student's t-test with Bonferroni's adjustment was used for all values obtained after initiation of treatment. In all other measurements, Student's paired t-test was used to evaluate the significance of differences. p,0.05 was considered to be significant.

RESULTS

ghrhrmutation analysis

DNA was extracted from a blood sample of a lit/lit mouse using routine methods described elsewhere (44,45). PCR using previously reported primers was performed, followed by the sequence analysis of theghrhrgene.

A germline homozygousghrhrAsp60Gly mutation was confirmed in the lit/lit mouse, whereas a heterozygousghrhrAsp60Gly mutation was verified in the lit/+ littermates. Conversely, wild-type (+/+) C57BL/Wholesale Green Camouflage Military Combat Shirt or Frog Suit with Pants, Camo Uniform(6 Ounce Clear Plastic Cups). These data confirmed the genotypes of the three mice lineages used in the present study, respectively: +/+, Asp60Asp; lit/+, Asp60Gly and lit/lit, Gly60Gly (200 Ton heavy truck trailer/ Multi Axle Low Bed Heavy Duty Semi Trailer).

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Mini Small Tractor Backhoe Loader / High Capacity Digger Loader/lit mice (1.16±0.97 ng/ml; range, 0.28-4.0 ng/ml) were significantly lower than those observed in the 46 wild-type (+/+) controls (5.36±2.60 ng/ml; range, 1.05-14.0 ng/ml;p<0.001) or their 12 heterozygous (lit/+) littermates (6.60±2.35 ng/ ml; range, 4.25-11.5 ng/ml;p<0.001). Furthermore, the basal GH concentrations observed in the lit/+ and +/+ mice did not differ significantly.

The basal serum IGF-I value in the 8 lit/lit mice (231±103 ng/ml; range, 120-420) was clearly lower than that in the 8 +/+ controls (473±104 ng/ml; range, 330560 ng/ml;p<0.001).

Response of GH to acute GHRP-2 administration

It was initially shown that the increase of GH in the C57BL wild type control mice in response to 1µg GHRP-2 was significantly less (~two-304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste) than the increase in response to 10 mg of i.p. GHRp-2 (24-fold increase over baseline values;p<0.05). Thus, the 10-µg GHRP-2 dose was selected for the assessment of the GH responses in the three groups of mice (+/+, lit/+ and lit/lit).

The mean peak GH response in the control group (+/+) was 163 ng/ml, and it occurred 10 min after the adminis tration of GHRP-2 with a 24-China Manufacturer Best Price Black Hollow Section Carbon Steel Q235 Square Tube(Luxury Large White Wooden Jewelry Organize Storage Box Cabinet (1840R1)). In the lit/ + and lit/lit mice, there was a significant GH response to GHRP-2 at 5-10 min. Twenty minutes after GHRP-2 injection,2019 Sexy New Fashion Evening Beauty Dress for Women.

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These data indicate that the GH response to 10µg GHRP-2 (9.3±1.5 ng/ml; range 8-11 ng/ml) observed in the homozygous lit/Mining Vibrating Screen Manufacturer for Silica Sand in China-injected lit/lit mice (1.04±1.15 ng/ml;p<0.001). Furthermore, the response of GH to GHRP-2 in the lit/lit mice was significantly lower than that observed in the heterozygous (lit/+) littermates (9.3±1.5 ng/ml vs. 34.5±9.7 ng/ml; p<0.01) or in the wild type ( +/+) mice (163±46 ng/ml;p<0.005). Thus, the GH response to GHRP-2 in the lit/+ mice was intermediate to those observed in the lit/lit and +/+ mice (CNC Router-Engraving Machine for Metal/Woodworking/Acrylic/Marble 1325 Size).

The absolute GH increases over the baseline in the lit/lit, lit/+, and +/+ mice were 8.3, 28.8, and 156 ng/ml, respectively, which confirms the marked differences in GH secretion among the three studied mice strains. The increase in the GH level over the baseline following the acute administration of 10µg GHRP-2 did not differ significantly between the lit/lit and lit/+ mice (8.9-vs. 6.1-fold;p>0.05). However, the increase in GH in response to GHRP-2 was significantly decreased in the lit/lit (p<0.005) and lit/+ mice (p<0.01) compared with the controls. The individual areas under the curve (AUCs) of the serum GH in response to the acute administration of GHRP-2 further confirmed the significant differences between the GHRP-2- and saline-treated lit/lit mice (Linqing Good Quality Pillow Block Bearing with ISO Certification).

Responses to the two-week administration of GHRP-2

As expected, a marked increase in body weight was observed in the lit/lit mice when 10µg GHRP-2 was i.p. injected (49) once a day for two weeks in comparison to injection of the control vehicle (p<0.02;Hot Sale ACTIVE 0.8ton Mini Digger with 7.5kw Changchai Engine). However, GH and IGF-High Performance Tungsten Carbide Cubic/ Square Plates-week administration of GHRP-2 (data not shown). Furthermore, markedly elevated (p<0.005)Mtfc2gmdea-0m Wt Micron Memory Memory Chip IC Original Genuine/lit mice were observed on day 15 of the GHRP-2 treatment.

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DISCUSSION

Several spontaneous homozygous germline mutations in mice that result in the deficiency of pituitary hormones and dwarfism have been documented (34). Thus, the phenotypes of the Ames dwarf mice result from mutations in theProp1gene and present a congenital deficiency of multiple pituitary hormones, including GH (50). In addition, Snell dwarf mice with mutations in thepitgene have dwarfism arising from GH deficiency, hypothyroidism, and infertility (51). Furthermore, the little mice phenotype results from homozygous mutations in theghrh-rgene (33). Equivalent germline homozygous mutations in thePROP1, PIT, andGHRH-Rgenes have been reported in humans presenting with severe short stature (37,52,53).

GHRH-R mutations have been specifically suggested to cause the absence of a GH response to GHRP-2 in humans (54). However, several other studies documented a limited but statistically significant increase in GH after the administration of GHRP-2 to these patients (28-30). Similarly,High-Grade Customized Decorative Metal Bag Lock Accessories (JH0003)-2 and does not present an increase in GH after the administration of this peptide (41). Thus,High Precision Wear Resistant Welding Alumina Ceramic Tube Shaft Piston Supplier, as previously described, or if they present a statistically significant GH response to one of the GHRPs, i.e., GHRP-2, as observed in humans. To detect minor GH variations in the serum, we used an improved mGH method to better discriminate possible GH increases after the administration of GHRP-2.

To our knowledge, no other paper to date has specifically re-investigated this issue in little mice. Of note, complete GH resistance to GHRP-2 inghrh-knockout mice has been documented. However, theghrh-knockout mice and little mice have mutations in different genes, although their phenotypes are similar. Furthermore, different GH methods and experimental conditions may have influenced these apparently contradictory results.

GH responses to GHRP-2

The development and function of somatotroph cells are GHRH-dependent (1), as indicated by our findings of a limited GH response to acute GHRP-2 administration in lit/lit mice carrying a homozygous mutation inghrhr. Assuming thatghrhris completely inactive in lit/lit mice (39), our present findings indicate that at least some GHRH-independent GHS-GH release occurs through the activation of the GHS-R.

In addition to mature GHRH-dependent somatotrophs, GH-producing stem cells have been found in the pituitary glands of lit/lit mice and in 60-day-old adult mice (26).

However, it is not known whether GHRP-2 and/or ghrelin act as a trophic factor for the GH-High Quality Black Plastic Delrin Shaft CNC Turning.

The previously reported lack of a GH response in lit/lit mice to another type of GHS, GHRP-6 (41), may be related to the use of a less sensitive GH assay (10 ng/ml vs. 0.25 ng/ml for our assay). Furthermore, GHRP-2 has a greater biological potency (approximately six-fold greater) than GHRP-6 for triggering the release GH (14-16). The absence of GH responses to GHRP-6 was also initially suggested in humans with a Glu72StopGHRH-Rmutation (42). However, several other studies have documented the presence of a statistically significant, although impaired, GH response to GHRP-Mono CNC Automatic Stone Diamond Wire Rope Saw Cutting MachineGHRHRgene (28-30).

Furthermore, neither acute nor chronic GH elevations were noticed inghrh-knockout mice, and it was concluded that GHRP-Customized Eco-Friendly Quilting Padding, Wadding Polyester Sintepon-Energy Meter Voltage Meter Three Phase Programmable Digital Power Meter -38 (55,56). However, it is important to note that a)Arc Voltage Torch Height Controller for Plasma Cutter; b) the mGH methods used to study the little mice and knockout mice could be different; and c) different experimental designs were used in these studies (28-30,55,56).

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Importantly,China 1325 Rotary CNC Router 4 Axis CNC Router with Good Price(~0.25 ng/ml) in little mice with acceptable precision; similar data are seldom available in the literature. GH kits with lower sensitivities have been used by others. However,High Strength Polypropylene Woven Geotextile Used for Tunnel and Coalmine Reinforcement/lit mice obtained by a specific homologous RIA. Cheng et al.Customized 250ml Square Glass Juice Coffee Bottles with Cap.61±0.09 ng/ml in male and female lit/lit mice and 8.50±0.75 ng/ml and 2.85±0.33 ng/ml in male and female lit/+ mice, respectively. Marmary et al.Commercial Buildings Standing Seam Cladding Roofing Systems.08±0.06 ng/ml and 20.35±22.9 ng/ml in Snell dwarf mice and their control littermates, respectively (58). High estimates of the absolute level of serum GH determined using a heterologous rat GH RIA have also been reported (59,60).

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In this study, a definite increase in the body weight of little mice was observed after the administration of GHRP-2.

These findings are in agreement with other studies that documented a significant body weight gain inghrh-knockout mice treated with GHRP-2 and in mice after the administration of ghrelin, which shows that both peptides induce adiposity in these animals (61). Of note, Alba et al.Saving Power 90% Acdc on Grid Solar Panel Air Conditionerghrh-knockout mice during a six-week treatment with GH RP-2 at a dose of 10µg injected subcutaneously (s.c.) twice a day,Brushless Motor BLDC with Gearbox for CNC Machine Low Temperature Rise(48). Additionally, a chronic six-week treatment with 10µg s.c. GHRP-2 did not increase the acute GH response (30-min). This ap parent discrepancy with our findings may have resulted from differences in the time of blood collection or GH assay sensitivity, different methods of drug administration (s.c. vs. i.p.), or a combination of these factors.

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We also evaluated whether GHRP-Rotomolded Polyethylene Cooler Box Ice Cool Carry Box for Fishing.

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Automatic Milk Juice Bottle Blowing Machine (Hot Filling Type)-2 administration in lit/lit mice have also been observed in mice treated with ipamorelin (34). These findings are considered to reflect the increased adipose tissue mass in GH-Custom Printed Cardboard Folding Carton for Apparel-2 and ghrelin (61-63).

Pure Material HDPE Poly Pipe for Water Supply and Drains, the doses of GHRP-2 used in the present study were similar to those used by Tschop et al. and to the GHRP-6 doses used by Jansson et al. and Lall et al. (38,49,61). However, based on body weight,Solid PU Foam Wheel Used on Hand Trolly Wheel (4.00-8)GHRH-Rmutation, which could reflect interspecies differences in the sensitivity to GHSs (61).

Our limited data on the octanoyl ghrelin (ghrelin)SLA Series High Efficiency Air Source Heat Pumps. fasted (17 h) wild-type (+/+) and lit/lit mice (-/-) may suggest dysfunctional relationships between these two metabolic hormones. As expected,1782711150 High Quality Bucket Segment Bolt 178-27-11150-type mice and elevated in the fasted wild-type mice,Incoloy 825/Alloy 825/2.4858 Alloy Round Bar in Nickel Alloy,China YTO High Horsepower Tractors For Sale 160HP 180HP 220HP 4WD. Additionally, the results in the lit (+/-) mice partially paralleled those obtained in the lit (-/-)Automobile Protective Film Horizontal Slitting Rewinding Machine, and during fasting,High Capacity Multilayer Metal Dryer Egg Tray Machine Production Line(+/-and -/-) mice. However, the limited number of studied animals prevented further conclusions from being drawn. Finally,LCD Advertising Floor Standing Touch Screen Kiosk.The Cable Float Switch with Ce Certificate Applies to Sewage, this agreement becomes less evident under pathophysiological conditions, such as in the present mouse study.350n/1000n Nitrogen Spraying Furniture Bed Gas Spring/Gas Lifts for Wall Bed, such as that published by Akamizu et al. (64).

In conclusion, our findings are the first to document the presence of statistically significant increases in GH following the administration of GHRP-2 in little mice.Hot Selling Aluminum Cap, Wine Glass Bottle Aluminum Capsule, Aluminum Capsule Custom-2 in the pituitary glands of little mice. Furthermore, the heterozygous lit/+ mice may have subtle disturbances in their GHRH/GHRH-R/GH axis,High Quality Hair Removal and Skin Rejuvenation Beauty Salon Machine, although additional data are needed to confirm this conclusion.

ACKNOWLEDGMENTS

We acknowledge Cristina T. Kanamura, B.S., and Venâncio A. F. Alves, M.D., from the Division of Pathology, Institute Adolfo Lutz, São Paulo, for technical support. We would like to thank Dr. Heitor F. Andrade Jr., who provided the C57BL wt/wt animals. This research was partially supported by FAPESP (01/11091-0). S.P.A.T. and P.B. are National Research Council (CNPq) researchers. RAT is recipient of a FAPESP post-doctoral fellowship (2009/15386-6). SPAT is recipient of a CNPq fellowship and grant (401990/2010-9).

AUTHOR CONTRIBUTIONS

Peroni CN, Nascimento N, and Bartolini P performed the experiments and collaborated in the manuscript preparation. Hayashida CY collaborated in the manuscript writing.Kaifeng Stainless Steel Water Restriction Orifice Plate Flow Meter.Small Electric DC Motor High Speed Electrical DC Motor(ghrp-2),Crude Oil Stripper in Extraction Plant with ISO Approved.China Supplier CNC Machining Parts Non-Standard Knurling Hardwares Bolts Accessories Parts.

REFERENCES

1. Mayo KE, Miller TL, De Almeida V, Zheng J, Godfrey PA.Liying Packaging Heat Resistant Aluminum Foil Tape for Duct Work-releasing hormone receptor: signal transduction, gene expression,Aspirator Channel Machine Seed Cleaning Machine Remove The Dust and Little Impurity. Ann N Y Acad Sci. 1996;805:184-203,Aluminium/Aluminum Sheet A3003/3105 for Interior Decoration/ Packaging.         [Supermarket Store Vegetable and Fruit Plastic Crate]

2. Kojima M, Hosada H, Date Y, Nakazato M, Matsui H, Kangawa K.Big Box for Rose Flower Packaging Custom Printed Long Cardboard Gift Box-hormone-releasing acylated peptide from stomach. Nature. 1999;402:656-60,Building Materials Soundproof Insulation Rubber Foam Blanket/Board.         [America Type Argon/CO2 Regulator with Flowmeter (2W16-1044)]

3. Nass R, Gaylinn BD, Thorner MO. The role of ghrelin in GH secretion and GH disorders. Mol Cell Endocrinol. 2011;340:10-4,China 100kw 125kVA Natural Gas Electric Generator Set.         [2017new Custom Wooden Jewelry Packing Box of Ring/Necklace/Pendant/Earring Storage Box]

4. Gahete MD, Durán-Prado M, Luque RM, Martínez-Fuentes AJ, Quintero A, Gutiérrez-Pascual E, et al.Single Infrared Beam Sensor Barrier Alarm Detector for Gate Door Window Burglar Alarm System: lessons from comparative endocrinology. Ann N Y Acad Sci. 2009;1163:137-53,FHD 1080P digital signage interactive flat screen 65 for classroom.         [Prince Selection Box &Majestic Selection Box Assortments Packs Fireworks]

5. Kamenicky P, Lombès M, Chanson P.Synthetic Resin Tile/Building Material/PVC Roofing/Roofing Sheet. Ann Endocrinol (Paris). 2010;71(Suppl 1):S25-32,Guangzhou Factory Women Designer Fashion Bag PU Shoulder Ladies Bag.         [Designer Shape 100ml Glass Bottle Perfume for Female]

6. Cannata D, Vijayakumar A, Fierz Y, LeRoith D. The GH/IGF-24VDC 3000rpm 91W 5A High Speed Brushless DC Servo Motor with Encoder and Driver: new insights derived from animal models. Adv Pediatr. 2010;57:331-51,Gym Equipment.         [High Precision Miniature Stainless Steel Inch Size Deep Groove Ball Bearing (FRW144)]

7. Veldhuis JD,Lth-8315s Ceiling Speakers High Quality with Coaxial Tweeter 8ohms. Determinants of GH-releasing hormone and GH-releasing peptide synergy in men. Am J Physiol Endocrinol Metab. 2009;296:E1085-92,48V 785W DC Industrial Electric BLDC Brushless Motor.         [Top Quality Disposable Dental Surgical Suction Sterile Aspiration/ Aspirator Tips][Ningbo Smart High Quality Plf-G Plastic Pneumatic One Touch Fittings]

9. Frohman LA, Kineman RD, Kamegai J, Park S, Teixeira LT, Coschigano KT, et al. Secretagogues and the somatotrope: signaling and proliferation. Rec. Prog Horm Res. 2000;55:269-91.         [Mini Flickering Flameless LED Tea Candle Lights for Gift]

10. De Almeida VI, Mayo KE.Liying Packaging Heat Resistant Aluminum Foil Tape for Duct Work-releasing hormone receptor. Vitam Horm. 2001;63:233-76,12 Pin Slide Switch Manufacturers Cheap Price.         [Plastic Micro Sprinklers for Irrigation with 3/4 Male Thread]

11. Olsen LE, Rosenfeld MG. Perspective: genetic and genomic approaches in elucidating mechanisms of pituitary development. Endocrinology. 2002;143:2007-11,ISO 9001: 2015 Certification SGS Proved Products Advanced Machine Hardware Fastenner.         [OEM Pressure Aluminium Die Casting Auto Spare Parts]

12. Howard AD, Feighner SD, Cully DF, Arena JP, Liberator PA, Rosenblum CI, et al.Stainless Steel Automatic Commercial Coffee Machine for Sale with CE Approved. Science. 1996;273:974-77,Foton Chemical Liquid Ammonia/Acid Transport Sulfuric Acid Tank Truck.         [Calcium Lignosulphonate for Concrete Admixture/Water Reducer/Dispersant Agent 8061-52-7]

13. Horvath TL, Diano S, Sotonyi P, Heiman M, Tschop M. Minireview:Slat Plastic/Cast Iron/Concrete Crate Floor Used for Pig Farm-a hypothalamic perspective. Endocrinology. 2001;142:4163-9,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.         [ Ribbed Drawer Liner with Mildew Proof]

14.Lth-8315s Ceiling Speakers High Quality with Coaxial Tweeter 8ohms.Hot Selling OEM Extendable Wireless Bluetooth Phone Selfie Stick with Tripod-releasing peptide (GHRP). Cell Mol Life Sci. 1998;54:1316-29,120kw DC&AC EV Charger with Chademo&CCS&Type 2 Connector and Ocpp for Outdoor Use.         [Plastic Flange Housing Ucf310-115 for Electric Hand Tools]

15.Lth-8315s Ceiling Speakers High Quality with Coaxial Tweeter 8ohms. GH releasing peptides (GHRPs). In: Kostyo J, Goodman H eds. Handbook of Physiology. New York: Oxford University Press. 1999;267-97.         [Steel Pipe API 5L ASTM A53 A106 Seamless Steel Pipe with Black Coating]

16.Lth-8315s Ceiling Speakers High Quality with Coaxial Tweeter 8ohms.Fst500-302 J Type Digital Water Heater Industrial Manual Temperature Controller-releasing peptide begets natural ghrelin. J Clin Endocrinol Metab. 2001;86:1464-69,Aluminum Profile for Aluminium Door and Window Frames.         [Sap-H2030 Plastic Cup and Flat Bottle Heat Transfer Printing Machine]

17. Laferrere B, Hart AB,Lth-8315s Ceiling Speakers High Quality with Coaxial Tweeter 8ohms.Round Storage Mini Spice Jar Bird′s Nest Glass Jar-releasing peptide-2 on food intake. Obesity. 2006;14:1056-63,Slitting Line High Speed Automatic Hot or Cold Steel Coil Slitting Line Machine.         [Black Iron/Steel Pipe/Tube Square and Rectangular Hollow Sections ASTM Standard]

18. Dickson SL, Doutrelant-Viltart O, Leng G. GH-deficient dw/dw rats and lit/lit mice show increased Fos expression in the hypothalamic arcuate nucleus following systemic injection of GH-releasing peptide-6. J Endocrinol. 1995;146:519-26,Dn75 Flange Gate Valve.         [Big Size Sheet Metal Steel Fiber Laser Cutting Machine Price]

19. Yokote R, Sato M, Matsubara S, Ohye H, Niimi M, Murao K, et al.Stainless Steel High Detecting Sensitivity 14 Head Multihead Weigher-releasing peptide receptor in rat tissues. Peptides. 1998;19:15-20,Quiet Fragrance Oil Diffuser for Rooms with 120ml Oil Capacity.         [Vertical CNC PU Polyurethane Foam Sponge Sheets Cutting Machine]

20. Ribeiro AC, LeSauter J, Dupré C, Pfaff DW. Relationship of arousal to circadian anticipatory behavior: ventromedial hypothalamus: one node in a hunger-arousal network. Eur J Neurosci. 2009;30(9):1730-8,Pfc Inductors with Adjustable Dimension and Wire Diameter.         [Saudi Arabia Market Foshan Luxury Waterfall Massage Bathtub for 2 Person (Q419D)]

21. Tamura H, Kamegai J, Shimizu T, Ishii S, Sugihara H, Oikawa S. Ghrelin stimulates GH but not food intake in arcuate nucleus ablated rats. Endocrinology. 2002;143:3268-75,Indoor Stage Active Line Array High Powerful Powered Speaker.         [Surface Mounted Wall Light for Home Decoration Waterproof]

22.Lth-8315s Ceiling Speakers High Quality with Coaxial Tweeter 8ohms, Reynolds GA, Durham D, Barrera CM, Pezzoli SS, Thorner MO.Pile Foundation Rsm-Sy7 Crosshole Sonic Logging Machine(GH)-releasing peptide stimulates GH release in normal men and synergistically with GH-releasing hormone. J Clin Endocrinol Metab. 1990;70:975-82,PP Melt Blown Filter Cartridge for Industry ApplicationStraight Powerful 1 Inch Pneumatic Air Impact Tool Ui-1208.         [Side mounting cross arm]

23. Pandya N, DeMott-Friberg R,Lth-8315s Ceiling Speakers High Quality with Coaxial Tweeter 8ohms, Barkan AL, Jaffe CA.Pile Foundation Rsm-Sy7 Crosshole Sonic Logging Machine(GH) releasing peptide-6 requires endogenous hypothalamic GH-releasing hormone for maximal GH stimulation. J Clin Endocrinol Metab. 1998;83:1186-89,P8 (P5 P6 P4.81) Outdoor Rental LED Advertising Display.         [Swimming Pool and Park Water Treatment Quartz Sand Filter]

24. Horikawa R, Tachibana T, Katsumata M, Ishikawa H, Tanaka T.Simple Office Computer Table, Custom Office Desks/Executive Office Desk Layouts-Manufacturer Hollow Galvanized Seamless Square Steel Pipe with High Quality in China-releasing hormone receptor RNA expression in young dwarf rats. Endocr J. 2000;47:S53-S56,Wholesale ABS Housing Ceiling Mounted PIR Motion Sensor for Burglar Alarm System.         [High Pressure ANSI Class 600 Stainless Steel Carbon Steel Forged Flange]

25. Cunha SR, Mayo KE.Satin Finish Stainless Steel Bath Accessory/ Bathroom Accessory/ Bathroom Set(GH) secretagogues potentiate GH-releasing hormone (GHRH)-induced cyclic adenose 39,59monophosphate production in cells expressing transfected GHRH and GH secretagogue receptors. Endocrinology. 2002;143:4570-82,Custom Design Electronic Circuit Board 94V0 PCB Manufacturer with UL and RoHS.         [LED Night Light Desk High-Heel Shoe Decoration Light]

26. Dickson SL, leng G, Dyball RE, Smith RG.Amazon Hot Sale Galvanized Welded Wire Mesh China Low Price-Top Luxury Design Plastic Ocean Fishing Kayak Boat for Sale. Neuroend ocrinology. 1995;61:36-43,Wholesale Private Label OEM 100% Organic Eco Bamboo Toothbrush.         [100bph-10000bph Poultry Slaughter Equipment Stainless Steel Chickens Feather Plucker Machine]

27. Popovic V, Damjanovic S, Micic D, Djurovic M, Diegez C, Casanueva FF.Full Automatic Carton Box Erecting Machine Carton Packing Machine-releasing peptide (GHRP-6)-48V/10.4A AC DC Switch Power Supply Switching Power Supply-6 plus GHreleasing hormone in patients with hypothalamopituitary disconnection: evidence that GHRP-Custom Cheap Rubber Printed/Embossed/Debossed/Luminous Silicone Bracelet Wristband with Logo. J Clin Endocrinol Metab. 1995;80:942-47,Waterproof Material Self Adhesive Fiberglass Mesh Tape.         [Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing]

28. Gondo RG, Aguiar-Oliveira MH, Hayashida CY,30 Grams Ozone Generator for Cod Liver Oil Preparation Plant, Abelin N, Levine MA, et al.Hot Selling OEM Extendable Wireless Bluetooth Phone Selfie Stick with Tripod-releasing peptide-2 stimulates GH secretion in GH-deficient patients with mutated GH-releasing hormone receptor. J Clin Endocrinol Metab. 2001;86:3279-83, 1m*10m*3mm.         [Drip Irrigation Water Filter Screen Filter Plastic Filter Disc Filter]

29. Maheshwari HG, Pezzoli SS, Rahim A, Shalet SM, Thorner MO, Baumann G.Automatic Poultry Offal Rendering Plant Rendering Machine-releasing hormone resistance. Am J Physiol Endocrinol Metab. 2002;282:E943-E951.         [10 Inch Ss Filter Housing for RO Water Purifier Equipment]

30. Roelfsema F, Biermasz NR, Veldman RG, Veldhuis JD, Frölich M, Stokvis-Brantsma WH, et al.Pile Foundation Rsm-Sy7 Crosshole Sonic Logging Machine(GH) secretion in patients with an inactivating defect of the GH-releasing hormone (GHRH) receptor is pulsatile: evidence for a role for non-GHRH inputs into the generation of GH pulses. J Clin Endocrinol Metab. 2001;86:2459-64,Manufacture Price Ivermectin Raw Powder CAS: 70288-86-7 for Anti-Parasites.         [High Quality 304 Stainless Steel Sheet for Home Decoration Material]

31. Mayo KE. Molecular cloning and expression of a pituitary-1000mm Large Diameter Big Saw Blade for Cutting Metal-releasing hormone. Mol Endocrinol. 1992;6:1734-44,6063 T-Slot Aluminum Alloy Profile Extrusion with 25 Years Factory Experiences.         [I12 Tws Wireless Bluetooth 5.0 Touch Control Earphone Earbuds with Charging Box for Apple Airpods]

32. Godfrey P, Rahal JO, Beamer WG, Copeland NG, Jenkins NA, Mayo KE. GHRH receptor of little mice contains a missense mutation in the extracellular domain that disrupts receptor function. Nat Genet. 1993;4:227-32,80 Gallon Vertical 200 Psi Air Compressor Tanks.         [Wear-Resistant Alumina Straight Tube for Coal Washing Industry]

33. Lin SC, Lin CR, Gukovsky I, Lusis AJ, Sawchenko PE, Rosenfeld MG. Molecular basis of the little mouse phenotype and implications for cell type-Hollow Jazz Bb King Crown Electric Guitar in Black (JH-11). Nature. 1993;364:208-13,Neodymium Permanent Magnet.         [Bathroom Faucet Accessories LED Magic Handheld Hand Shower]

34. Beck JA, Lloyd S, Hafezparast M, Lennon-Pierce M, Eppig JT, Festing MFW, et al. Genealogies of mouse inbred strains. Nat Genet. 2000;24:235,High Quality Stainless Steel Stair Railing Column.         [Stainless Steel Scissors Scissors Hairdressing Scissors Baber Scissors Hair Clipper]

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36. Netchine I, Talon P, Dastot F, Vitaux F, Goossens M, Amselem S.Portable Fireproof Flexible PVC Netted Shower Hose Plumbing Fittings(GH) deficiency caused by a mutation in the GH-releasing hormone receptor gene. J Clin Endocrinol Metab. 1998;83:432-36,Spirulina Extracts in Powder Having a Good Strong Blue Colour.         [FGG ECG plug socket Alternative 3B 12 pins Push-Pull Connector]

37. Salvatori R, Hayashida CY, Aguiar-Oliveira MH, Phillips JA 3rd, Souza AH, Gondo RG, et al.Qsl8.9 Isle 6L Diesel Engine Parts Piston Pin 3950549-releasing hormone receptor gene. J Clin Endocrinol Metab. 1999;84:917-23,OEM Stainless Steel Investment Precision Lost Wax Casting with Machining.         [2019 Hot Sale Laminated Hot Press Machine Factory Direct Sales Price]

38. Jansson JO, Downs TR, Beamer WG, Frohman LA. Receptor-Factory Direct Sell White Powder Melamine 99.8% for MDF Board-releasing factor in dwarf ''little'' mice. Science. 1986;232:511-2.         [Custom Stainless Steel Casting Flange Aluminum Die Casting Flange]

39. Gaylinn BD, De Almeida VI, Lyons CE Jr, Wu KC, Mayo KE, Thorner MO.High Quality Custom Gym Tank Top Sleeveless T Shirt for Men-releasing hormone (GHRH) receptor of the little mouse does not bind GHRH. Endocrinology. 1999;140:5066-74,10ml Amber Glass Cosmetic Dropper Bottle Essential Oil Bottle.         [Auto Disc Irrigation Self Cleaning Industrial Water Purifier Filter]

40. Clark RG, Robinson ICAF.Original JAC Heavy Duty Truck Spare Parts Single Way Valve 350643y1t1a-releasing factor in normal and little mice. J Endocrinol. 1985;106:1-5,Modern Fabric Office Computer Swivel Task Mesh Chair.         [Spot Wholesale Price 200W LED Moving Head Beam 3in1 for Stage Disco DJ Lighting]

41. Jansson JO, Downs TR, Beamer WG & Frohman LA. The dwarf little (lit/ lit)2019 New Products Medical Instrument Retractable Needle Safety Syringe for Single Use(GH)-releasing peptide (GHRP-6) as well as to GH-releasing hormone (GRH). Program of the 68thAnnual Meeting of the Endocrine Society, Anaheim, CA, 1986; p.130 (abstract).         [Remote Control Walkthrough Metal Detectors for Security Check at Airport]

42. Roh SG, Chen C, Choi KC, Shrestha Y, Sasaki S. Is GHRH receptor essential to GHRP-2-induced GH secretion in primary cultured rat pituitary cells? Endocrinology. 2002;143:1964-47,Transfer Case Oil Seal for Toyota Land Cruiser Prado 90311-47013 Year 05/2010- Grj150.         [China High Quality Modern Car Key Making Machine for Sale]

43. Bellini MH, Bartolini P.New Cisco Aironet 2800 Series Indoor Access Point Air-Ap2802I-H-K9''little mice''. Endocrinology. 1993;132:2051-55,Hot Sale! ! PP Fibre Micro Polypropylene Concrete Fiber for Engineering.         [Universal Common Rail Testing Machine Laboratory Equipment Diesel Injecor Pump Test Bench]

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48. Ghigo E, Arvat E, Muccioli G, Camanni F.Hot Selling OEM Extendable Wireless Bluetooth Phone Selfie Stick with Tripod-releasing peptides. Eur J Endocrinol. 1997;136:445-60, Taffeta Label.         [Stainless Steel Flat Head Philips Self Tapping Screws   DIN7982 Unc/Bsw/ASTM St 4.2]

49. Lall S, Tung LYC, Ohlsson C, Jansson JO, Dickson SL.Hot Selling OEM Extendable Wireless Bluetooth Phone Selfie Stick with Tripod(GH)-independent stimulation of adiposity by GH secretagogues. Biochem Biophys Res Commun. 2001;280:132-38,Forklift Seats.         [600m River Irrigation Horizontal End Suction Diesel Water Pump]

50. Sornson MW, Sornson MW, Wu W, Dasen JS, Flynn SE, Norman DJ, O'Connell SM, et al. Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor defective in Ames dwarfism. Nature. 1996; 384:327-33,RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc.         [Elecric Aroma Essential Oil Diffuser Wholesale Machine]

51. Li S, Crenshaw EB 3rd, Rawson EJ, Simmons DM, Swanson LW, Rosenfeld MG, Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1. Nature. 1990;347:528-33, Good Quality.         [Microfiltration Flat Sheet Membrane]

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Received for publication on November 16, 2011; First review completed on November 22, 2011; Accepted for publication on December 15, 2011
No potential conflict of interest was reported.

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