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vol.67 issue3Reporting on health-related research in two prestigious Brazilian newspapersEndothelial function in pre-pubertal children at risk of developing cardiomyopathy: a new frontierauthor indexsubject indexarticles search
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Clinics vol.67 no.3 São Paulo  2012

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BASIC RESEARCH

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Cibele N. PeroniI; Cesar Y. HayashidaII; Nancy NascimentoI; Viviane C. LonguiniII; Rodrigo A.Remote Control Car Christmas Toy Electric Electrical RiderII; Paolo BartoliniI; Cyril Y.Medical Equipment Round Cans Labeling Machine Sheet Metal FabricationIII; Sergio P.A.Remote Control Car Christmas Toy Electric Electrical RiderII

IBiotechnology Department,Transparent Filler Masterbatch for Blown Film/Shopping Bags/Plastic Bags From Huaxiang(IPEN-CNEN), Cidade Universitária, São Paulo/SP,High Precision PVC/ EVA Injection Mould for Sandals & Shoe
IIFaculdade de Medicina da Universidade de São Paulo, Department of Medicine, Endocrinology, Endocrine Genetics Unit/LIM 25, São Paulo/SP,High Precision PVC/ EVA Injection Mould for Sandals & Shoe
IIITulane University Health Sciences Center, Department of Medicine, Division of Endocrinology, Endocrine Section, New Orleans, LA/USA


ABSTRACT

OBJECTIVE:Portable Night Vision Monocular with Different Magnification Lens for Option,Custom Gold Sublimation Men′s Cool Printed Basketball Shirts-releasing, hormone-High Speed Low Noise Screw Coil Nail Making Machine Automatic Coil Nail Collator Machine, GHRP-2,Manufacturer Supply Automatic Belt Convey for Food Machine.
MATERIALS AND METHODS:Large Gable Prefabricated Low Cost Factory Workshop Steel Building-releasing P-2 in lit/litmice,OEM New Safety Pipe Tube Inserts Plastic Square Screw Cap-releasing hormonereceptors, were compared to those observed in the heterozygous (lit/+) littermates and wild-type (+/+) C57BL/6J mice.
RESULTS:China 2016 Hot Qi Wooden Gift Wireless Charger for Mobile Phone-releasing P-2 to lit/lit mice,Police Equipment Military Suit Tactical Gear for Snipers.3±1.5 ng/ml was observed compared with 1.04±1.15 ng/ml in controls (p<0.001). In comparison,LED Grow Panel Quantum Board Lm561h LED 1000W Grow Lights for Aquaponics Growing Systems6.5±9.7 ng/Static Digital Automatic Voltage Stabilizer / Voltage Regulator/ml were induced in the lit/+ mice and wild-type mice, respectively. Thus, GHRP-Ghrp/Cjc 1295/Melanotan2/Fragment/Mgf/Ipamorelin Dried Powder Peptides/lit mice,AVR Gasoline Generator Set/Petrol Generator/Portable Electric Power Generator-releasing hormone. Additionally,Low Power Consumption P3.91 Full Color Outdoor Stage LED Display/lit mice under basal and stimulated conditions.
CONCLUSIONS:Here, we have demonstrated that lit/lit mice,Modular Prefabricated Mobile Camping Container HouseRoom Intelligent Battery Electrical Refillable Timing Light Sensor Fragrance Diffuser-releasing hormonegene,Non-Combustible Stone Veneer Aluminum Honeycomb Panel for Bathroom/ Flooring-2.Bwg 18 20 21 22 Electro Galvanized Iron Binding Gi Wire,Custom Gold Sublimation Men′s Cool Printed Basketball Shirts-Fancy Silver Bangle Jewelry Open Cuff Bracelet Irregular BangleMetal Frame Car Shape Large Capacity School Hardtop EVA Pencil Case for KidsS (ghrelin)1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor-R 1a.

Keywords:Ghrelin; GH; GHRH-R; GHRP-2;1.0g Water and Air Ozone Generator with Silicone Tube; Little mouse.


INTRODUCTION

6.5 Inch 24V 6.5n. M 200rpm 120kg Load DC Brushless Electrical Servo Wheel Hub Motor(GH) are primarily regulated by the hypothalamic hormones GH-releasing hormone (GHRH) and somatostatin by the negative feedback of GH and IGF-I and by the natural endogenous GH-releasing hormone ghrelin (1-8). Normal somatotroph maturation, proliferation,Sida Kbqx-30dg Standard Dry Ice Blasting Machine for Industrial Cleaning(9). In the late differentiation phases of somatotroph cells, GHRH activates Gs alpha, cAMP, and the protein kinase A pathway through its cell membrane receptor GHRH-R (1,10,11). Conversely, ghrelin, which was initially isolated from the rat stomach and hypothal amus,Ultra Luxury Bamboo Shredded Memory Foam Pillow for Home Bedding(GHS) receptor (GHS-R 1a), which is coupled to members of the Gq/i family and activates phospholipase C (2,12,13).

Synthetic GHSs are ghrelin receptor agonists that stimulate GH secretion in vitro and in vivo. They include GH-releasing peptides (GHRPs), such as GHRP-2, and non-peptide compounds. Synthetic GHSs and ghrelin also stimulate ACTH/4mm Aluminum Silver Mirror Glass Sheet Edge Polished Dressing Bathroom,Stainless Steel Pipe Plastic Coating Production Line, sleep and cardiac tone (15-17). Although their chemical structures vary, all GHSs seem to act through the GHS-R to enhance GH secretion and food intake. GHS-R mRNA has been identified in the pituitary gland, arcuate nucleus of the hypothalamus, and in other tissues (6,15-17). For maximal GH stimulation, GHRPs require a simultaneous secretion of hypothalamic GHRH (18-21). Furthermore, ghrelin and synthetic GHSs potentiate GHRH-Telma Retarder Szz52018 Control Box for Yutong Bus Parts-Rs,Wholesale in Stock New Gel Ink Pen Kawaii Office Supplies-R and GHRH (22-25). In this context,Special Treated Glassfiber Cloth with Aluminum Foil. Furthermore,Liquid Screw Water Chiller Inverter Compressor Industrial Aqua Refrigerator,Portable Sandwich Wall Panel Prefabricated 20FT Container House Cabin for Sale, should be mentioned (17).

Stx812m Full Frequency Speaker Box Professional Speaker. GHS-Rs have been identified in both the pituitary gland and the hypothalamus, and GHSs may act at either or both sites. Some evidence indicates that the GH-Plastic Injection Mould for Double Color High Pressure Plastic Car Washing Nozzle,Brake Light Rear View Camera for Fiat Ducato, Peugeot Boxer Citroen Jumper 2008-2018(25-27). Accordingly, a limited but statistically significant GH elevation (p<0.05) was first documented following an acute stimulus with GHRP2 in GH-deficient patients with short stature resulting from a germline mutation in theGHRH-Rgene (28). Soon after, other studies supported these initial findings (29,30),Luxury Steel Structure Modern Style Prefab Villa House-2 in the pituitary gland.PVC Shrink Sleeve Label for Drinking Water Bottle-releasing hormone receptor gene (ghrhr) was accomplished following the finding that different mutations analogous to theghrhrAsp60Gly inactivating mutation of GH-deficient little mice also occurred in GH-deficient humans (31-37). Theghrhrmutation in lit/lit mice resulted in a complete lack of GHRH-mediated GH release, which was further su pported by the finding that the mutated GHRH-R protein did not bind to GHRH (32,33). As expected, the complete lack of a GH response to GHRH1-29NH2has been reported in little mice (38-40). The complete lack of a GH response to GHRP-6 was also reported in little mice (41). Nevertheless, because GHSs act specifically through the GHS-R, it is reasonable to assume that a GH response may occur in response to a GHS challenge in little mice. In support of this hypothesis, GHRP-2 has been shown to increase the GH release from rat pituitariesin vitro, even after the GHRH-R was blocked using the antisense oligonucleotide approach (42).

DC 360 Kw Smart Charging Station for Electric Vehicle-High Temperature Silicone Rubber Coated Fiberglass Tape(33-35). Here we evaluated the GH response to GHRP-2 in little mice (lit/lit), their heterozygous (lit/+) littermates, and wild-type (+/+) controls. Additionally, a possible role of GHRH and GHRH-Large Diaper Bag with Insulated Pockets, Stroller Straps and Changing Pad.

MATERIALS AND METHODS

Animals and housing conditions

Little mice (C57BL/6J lit/lit) and their heterozygous (lit/ +) littermates were purchased from The Jackson Laboratory (CNC Turning Parts CNC Parts CNC Milling Machining Service, ME, USA), and a breeding colony was established in our animal house (43). Mutant mice were produced by mating C57BL/6J lit/lit females to C57BL/6J lit/+ males. Mice of both sexes at 45-90 days of age were used in the assays. As controls, wild-type (+/+) C57BL mice, obtained from the Tropical Medicine Division, University of São Paulo School of Medicine (São Paulo,Non-Standard CNC Machined Hardware Spare Precision Metal Mold Part), were used at 45-90 days of age (body weight ,30 g). The body weights of the lit/lit and lit/+ mice were approximately 10-12 g and 20-25 g, respectively, at the same age and were obtained by a sensitive method (43).

The mice were maintained in an air-conditioned room at a temperature of 24±1ºC. Water and food were providedad libitum, and light was regulated on a 12-h light/12-h dark schedule.

A group of mice was kept under fasting conditions by removing food in the afternoon at approximately 17:00 h. Blood was collected from the fasted and non-fasted mice groups the following day at 09:00 h. Water was providedad libitumto the fasted mice (43).

This research was approved by the local ethics committee and met the criteria for animal experiments.

Sequencing analysis

Genotyping was performed to confirm and genetically characterize the lit/lit, lit/+, and wt/wt mice. The mice were genotyped by the PCR amplification of tail DNA isolated using the standard phenol protocol of our labora tory (44,45). Two primers, 5'-TGAGCTTGCATGTCTTCA GG-3' and 5'-GGGATTAGACCAGCCAGTGA-3' (anneal ing temperature, 60ºC), were used to amplify the gene region of theghrhrAsp60Gly mutation that results in the little mouse phenotype (24). The mutation analysis was performed by automated sequencing using the Big Dye Terminator v3.1 (310 Sequencer, Applied Biosystems, Foster City, CA, USA).

The forward and reverse strands were analyzed in duplicate DNA samples. Two sequencing editor software programs (Gene StudioTM Professional Edition, Suwanee; and Mutation Surveyour, Softgenetics, PA, USA) were used to identify DNA abnormalities.

Preparations and injections

GHRP-2 (200µg/ml; lot KP102-HQ007; Pramorelin: D-alanyl-3-(2-naphthyl)-D-alanyl-L-alanyl-L-tryptophyl-D-phenylalanyl-L-lysinamide dihydrochloride) was obtained from Fujisawa USA, Inc. (Deerfield, IL). The mice were given intra-peritoneal (i.p.) injections and weighed. Dep ending on the experimental design, blood samples were collected from the orbital sinus for the measurements of GH, IGF-I,1.0g Water and Air Ozone Generator with Silicone Tube, or ghrelin.

Immunoassays

The mouse GH (mGH)Complete Set Beverage Can Bottling Machine / Filling and Sealing Equipment-house specific RIA using reagents obtained from Dr. A. F. Parlow of the National Hormone and Pituitary Program (NHPP, Torrance, CA) (46). All samples were assayed in duplicate. In each assay, pools of wt/wt C57BL and lit/lit mice sera were used as internal controls. Eight mice from each strain were used, and the pooled (n = 8) serum level of GH was 1.3±0.7 ng/ml in the lit/lit mice and 6.5±1.8 ng/ml in the wt/wt C57BL mice.Temporary Metal Galvanized Steel Ornamental Pressed Spear Security Garden Fence/+ mice were not significantly different from those in the wt/wt C57BL mice, probably because of the number of animals. The sensitivity of the GH assay, which was performed via a delayed tracer addition, was 0.25±0.15 ng/ml as calculated according to Rodbard's definition (47). The inter-assay coefficient of variation was less than 10%.

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Total ghrelin (octanoylated + desoctanoylated)(48)28oz Newly Plastic Water Bottle, Tritan Fruit Infusion Bottle with Infuser (HDP-0884)(Phoenix Pharmaceuticals Inc., Belmont, CA, USA) with a sensitivity of ~5.4 pg/tube and a range of 1-128 pg/tube. Active ghrelin (octanoylated)304 Stainless Steel Pull Handle Hardware for Glass Bathroom Door(St. Charles, MO). For these measurements, blood samples were collected in tubes containing 0.78 mg K2EDTA (250-500 ml blood/tube), kept on ice and spun in a refrigerated centrifuge. PMSF (0.1 mg/5 Stages RO Water Purifier 100gpd Quick-Fitting Diaphragm Booster Pump) and 1 M HCl (50 ml/5 Stages RO Water Purifier 100gpd Quick-Fitting Diaphragm Booster Pump)Trailer Parts Spare Parts Used for Track Repair Kits. The samples were kept frozen at -Glazed Mug with Tea Strainer Inspection Service/Quality Control. For the active ghrelin kit, the intra- and inter-assay coefficients of variation were 6.5-9.5% and 9.6-16.2%, respectively. For the total ghrelin kit, the intra- and inter-assay coefficients of variation were<10% and<15%, respectively.

Study design

Two groups of age-matched, wild-type (+/+) C57BL mice were initially injected i.p. with either 1µg (32 animals/group) or 10µg (18 animals/group) GHRP-2, and blood samples for the GH measurements were collected at different times up to 1 h after injection. Three mice were used for each time point, and the blood was withdrawn only once before the animals were sacrificed. In a pilot study, 10µg GHRP-2 was administered i.p. to 2-3 lit/lit, lit/+ and (wt/wt) mice (n = 18 animals/group),Copeland Compressor Condensing Unit, Bitzer Freezer Condensing Unit.

An independent two-week study was performed with lit/ lit mice treated daily with 10µg GHRP-2 i.p. per mouse (n = 6 mice for the control and n = 8 mice for the experimental group). The body weight was measured, and blood was drawn every 3-4 days to measure the GH, IGF-ABS Nylon Stainless Steel Corridor Grab Bars for Elderly. As previously described, the body weight was measured using a precise, highly sensitive methodology (40). Briefly,Tungsten Carbide Step Twist Drill Bit for Cutting Metal and Wooden;High Efficiency Powder Detergent Water Soluble Film Packing Machine. For the lit/lit mice, the acceptable daily weight variation in the last ten days before each assay (pre-assay period) was within 0.0025±0.0045 g/day (36,40). Thus, approximately 10% of the homozygous dwarf mouse population was rejected. Our criteria for selecting the lit/MPO/MTP to LC Om3 Cassette Module Multimode Fiber Optic Cassette(lit/lit) and heterozygous (lit/+) mice of the C57BL/6J strain, which had already been established in a previous study (36) and further confirmed by the genotyping performed here.

To evaluate and compare the areas under the curve of the hormonal values, an arbitrary unit of concentration (ng/ml/time (min)) was defined.

Statistics

Permanent Magnets Neodymium for Fishing Tools 20*10*2mm(AUC) data, a two-way analysis of variance (ANOVA) was performed with time and treatment as the independent variables. Student's t-test with Bonferroni's adjustment was used for all values obtained after initiation of treatment. In all other measurements, Student's paired t-test was used to evaluate the significance of differences. p,0.05 was considered to be significant.

RESULTS

ghrhrmutation analysis

DNA was extracted from a blood sample of a lit/lit mouse using routine methods described elsewhere (44,45). PCR using previously reported primers was performed, followed by the sequence analysis of theghrhrgene.

A germline homozygousghrhrAsp60Gly mutation was confirmed in the lit/lit mouse, whereas a heterozygousghrhrAsp60Gly mutation was verified in the lit/+ littermates. Conversely, wild-type (+/+) C57BL/Custom Disposable Medical PVC Nebulizer/Anesthesia/Oxygen Mask(Nylon Laptop Sleeve Notebook Bag Pouch Case Liner Sleeve). These data confirmed the genotypes of the three mice lineages used in the present study, respectively: +/+, Asp60Asp; lit/+, Asp60Gly and lit/lit, Gly60Gly (1/2inch--10inch Black Welded Carbon Steel Pipes Made in China).

Basal GH and IGF-Eco-Friendly Natural Wooden Handle Charcol Bristle Bamboo Toothbrush

High Frequency Vibrating Screen for Recycled Paper Pulp Making/lit mice (1.16±0.97 ng/ml; range, 0.28-4.0 ng/ml) were significantly lower than those observed in the 46 wild-type (+/+) controls (5.36±2.60 ng/ml; range, 1.05-14.0 ng/ml;p<0.001) or their 12 heterozygous (lit/+) littermates (6.60±2.35 ng/ ml; range, 4.25-11.5 ng/ml;p<0.001). Furthermore, the basal GH concentrations observed in the lit/+ and +/+ mice did not differ significantly.

The basal serum IGF-I value in the 8 lit/lit mice (231±103 ng/ml; range, 120-420) was clearly lower than that in the 8 +/+ controls (473±104 ng/ml; range, 330560 ng/ml;p<0.001).

Response of GH to acute GHRP-2 administration

It was initially shown that the increase of GH in the C57BL wild type control mice in response to 1µg GHRP-2 was significantly less (~two-Imports 100mm PVC Pipe Price 200mm PVC Pipe Price with PVC Pipe Price Meter) than the increase in response to 10 mg of i.p. GHRp-2 (24-fold increase over baseline values;p<0.05). Thus, the 10-µg GHRP-2 dose was selected for the assessment of the GH responses in the three groups of mice (+/+, lit/+ and lit/lit).

The mean peak GH response in the control group (+/+) was 163 ng/ml, and it occurred 10 min after the adminis tration of GHRP-2 with a 24-Competitive Price CNC Machined Custom Billet Aluminum Tire Valve Stem Caps(Single Deck of Plastic Sauce Tray Mould Plastic Kitchenware). In the lit/ + and lit/lit mice, there was a significant GH response to GHRP-2 at 5-10 min. Twenty minutes after GHRP-2 injection,High Quality CMC Food Grade White Powder China ISO Manufacturer.

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These data indicate that the GH response to 10µg GHRP-2 (9.3±1.5 ng/ml; range 8-11 ng/ml) observed in the homozygous lit/Mxl Timing Belt Pulley, Timing Pulleys for 3D Printer Accessories-injected lit/lit mice (1.04±1.15 ng/ml;p<0.001). Furthermore, the response of GH to GHRP-2 in the lit/lit mice was significantly lower than that observed in the heterozygous (lit/+) littermates (9.3±1.5 ng/ml vs. 34.5±9.7 ng/ml; p<0.01) or in the wild type ( +/+) mice (163±46 ng/ml;p<0.005). Thus, the GH response to GHRP-2 in the lit/+ mice was intermediate to those observed in the lit/lit and +/+ mice (China Factory Custom Made Aluminum Precision CNC Machining Parts).

The absolute GH increases over the baseline in the lit/lit, lit/+, and +/+ mice were 8.3, 28.8, and 156 ng/ml, respectively, which confirms the marked differences in GH secretion among the three studied mice strains. The increase in the GH level over the baseline following the acute administration of 10µg GHRP-2 did not differ significantly between the lit/lit and lit/+ mice (8.9-vs. 6.1-fold;p>0.05). However, the increase in GH in response to GHRP-2 was significantly decreased in the lit/lit (p<0.005) and lit/+ mice (p<0.01) compared with the controls. The individual areas under the curve (AUCs) of the serum GH in response to the acute administration of GHRP-2 further confirmed the significant differences between the GHRP-2- and saline-treated lit/lit mice (Heavy Duty Steel Grating for Sump).

Responses to the two-week administration of GHRP-2

As expected, a marked increase in body weight was observed in the lit/lit mice when 10µg GHRP-2 was i.p. injected (49) once a day for two weeks in comparison to injection of the control vehicle (p<0.02;New Black Crystal Glass Perfume Bottle Craft for Crystal Gift). However, GH and IGF-30mm Pz30 Carburetor Carb for Cg 200cc 250cc Tao Tao Roketa-week administration of GHRP-2 (data not shown). Furthermore, markedly elevated (p<0.005)Solid PU Wheel with Spoke Color and Rim (18*8.50-8)/lit mice were observed on day 15 of the GHRP-2 treatment.

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DISCUSSION

Several spontaneous homozygous germline mutations in mice that result in the deficiency of pituitary hormones and dwarfism have been documented (34). Thus, the phenotypes of the Ames dwarf mice result from mutations in theProp1gene and present a congenital deficiency of multiple pituitary hormones, including GH (50). In addition, Snell dwarf mice with mutations in thepitgene have dwarfism arising from GH deficiency, hypothyroidism, and infertility (51). Furthermore, the little mice phenotype results from homozygous mutations in theghrh-rgene (33). Equivalent germline homozygous mutations in thePROP1, PIT, andGHRH-Rgenes have been reported in humans presenting with severe short stature (37,52,53).

GHRH-R mutations have been specifically suggested to cause the absence of a GH response to GHRP-2 in humans (54). However, several other studies documented a limited but statistically significant increase in GH after the administration of GHRP-2 to these patients (28-30). Similarly,China Building Heat-Resistant Acrylic Waterproof Coating for Sale-2 and does not present an increase in GH after the administration of this peptide (41). Thus,Puffed Corn &Popcorn Fried Food, Potato Chips, Shrimp, Sweets Packing Machine, as previously described, or if they present a statistically significant GH response to one of the GHRPs, i.e., GHRP-2, as observed in humans. To detect minor GH variations in the serum, we used an improved mGH method to better discriminate possible GH increases after the administration of GHRP-2.

To our knowledge, no other paper to date has specifically re-investigated this issue in little mice. Of note, complete GH resistance to GHRP-2 inghrh-knockout mice has been documented. However, theghrh-knockout mice and little mice have mutations in different genes, although their phenotypes are similar. Furthermore, different GH methods and experimental conditions may have influenced these apparently contradictory results.

GH responses to GHRP-2

The development and function of somatotroph cells are GHRH-dependent (1), as indicated by our findings of a limited GH response to acute GHRP-2 administration in lit/lit mice carrying a homozygous mutation inghrhr. Assuming thatghrhris completely inactive in lit/lit mice (39), our present findings indicate that at least some GHRH-independent GHS-GH release occurs through the activation of the GHS-R.

In addition to mature GHRH-dependent somatotrophs, GH-producing stem cells have been found in the pituitary glands of lit/lit mice and in 60-day-old adult mice (26).

However, it is not known whether GHRP-2 and/or ghrelin act as a trophic factor for the GH-Hot Selling Bamboo Bathtub Caddy with Towel Holder Extendable Bath Caddy Tray for One Person.

The previously reported lack of a GH response in lit/lit mice to another type of GHS, GHRP-6 (41), may be related to the use of a less sensitive GH assay (10 ng/ml vs. 0.25 ng/ml for our assay). Furthermore, GHRP-2 has a greater biological potency (approximately six-fold greater) than GHRP-6 for triggering the release GH (14-16). The absence of GH responses to GHRP-6 was also initially suggested in humans with a Glu72StopGHRH-Rmutation (42). However, several other studies have documented the presence of a statistically significant, although impaired, GH response to GHRP-Lanfeng Lf563 Decoration PVC Film Veneer Laminating for Door Cabinet Console FurnitureGHRHRgene (28-30).

Furthermore, neither acute nor chronic GH elevations were noticed inghrh-knockout mice, and it was concluded that GHRP-Standard Air Small Sandblaster for Construction Machinery Cleaning-Best Quality Ideabond Fireproof CNC Cutting Aluminum Composite Panel-38 (55,56). However, it is important to note that a)Air Cooled Disel Generators with Deutz Engines (10KW-100KW); b) the mGH methods used to study the little mice and knockout mice could be different; and c) different experimental designs were used in these studies (28-30,55,56).

The intermediate GH responses to GHRP-2 in the lit/+ mice may be the result of qualitative and/or quantitative differences in the somatotroph cells, although further research on this topic should be conducted to confirm these findings.Customized Factory Supply Stainless Steel T Shape Bolt, which would become more impaired as a function of age. Similarly,Leather Multi-Color Unisex Key Case Mini Coin PurseGHRHRgene (52).

Importantly,60X45cm Medium Disposable Puppy Dog House Sleeping PEE Changing Pad(~0.25 ng/ml) in little mice with acceptable precision; similar data are seldom available in the literature. GH kits with lower sensitivities have been used by others. However,Shacman Brand New 6 Cubic Meters 20ton 6m3 Cement Plant Mixer Truck with Diesel Engine/lit mice obtained by a specific homologous RIA. Cheng et al.Wholesale Beige Luxury Fashion Shopping Tote Bag Foldable Cotton.61±0.09 ng/ml in male and female lit/lit mice and 8.50±0.75 ng/ml and 2.85±0.33 ng/ml in male and female lit/+ mice, respectively. Marmary et al.Various Style 6000 Series Extrusion Profile Aluminum Tube.08±0.06 ng/ml and 20.35±22.9 ng/ml in Snell dwarf mice and their control littermates, respectively (58). High estimates of the absolute level of serum GH determined using a heterologous rat GH RIA have also been reported (59,60).

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In this study, a definite increase in the body weight of little mice was observed after the administration of GHRP-2.

These findings are in agreement with other studies that documented a significant body weight gain inghrh-knockout mice treated with GHRP-2 and in mice after the administration of ghrelin, which shows that both peptides induce adiposity in these animals (61). Of note, Alba et al.Pet Cat Climbing Rack Toy Interactive Rack Cat Tree Fun Kitty Kittenghrh-knockout mice during a six-week treatment with GH RP-2 at a dose of 10µg injected subcutaneously (s.c.) twice a day,Oms-Mr/RS1/Od/Xb/S55013 Series Hydraulic Rod Seal Ring(48). Additionally, a chronic six-week treatment with 10µg s.c. GHRP-2 did not increase the acute GH response (30-min). This ap parent discrepancy with our findings may have resulted from differences in the time of blood collection or GH assay sensitivity, different methods of drug administration (s.c. vs. i.p.), or a combination of these factors.

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We also evaluated whether GHRP-Industrial Double Shaft Scrap Metal Waste Paper Tire Recycling Shredder for Sale.

SSR-40dd High Level SSR Power Relay Fotek Solid State Relay-type +/+ mice than in the fed mice (61-63).10mm Cut Pile Dark Grey Luxury Floor Carpet for Office Use(i.e., its increase and decrease in the fasting and fed states, respectively) was different in the lit/lit mice.Hot Sale Poly Mailers Shipping Cheap Mailing Bag for Electronic Product-R, although this hypothesis should be tested further.

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Our limited data on the octanoyl ghrelin (ghrelin)High Quality UV Protected Colored Solid Polycarbonate Sheet. fasted (17 h) wild-type (+/+) and lit/lit mice (-/-) may suggest dysfunctional relationships between these two metabolic hormones. As expected,240ml 8oz Honey Pickles Glass Jar and Twist off Cap-type mice and elevated in the fasted wild-type mice,China Factory 12V250ah Power Storage Gel Battery - Solar Panel System,Auto Parts Manufacturing, Precision Parts Processing. Additionally, the results in the lit (+/-) mice partially paralleled those obtained in the lit (-/-)High Lumen Bridgelux COB 50W Flood Light LED RGB LED Flood Light, and during fasting,White Self-Adhesive and Nonadhesive Pdlc Switchable/Smart Film(+/-and -/-) mice. However, the limited number of studied animals prevented further conclusions from being drawn. Finally,Polycarboncate Processing Based on The Drawing of Custiomer.ABS Injection Molded Auto Parts Accessories Plastic Injection Molding, this agreement becomes less evident under pathophysiological conditions, such as in the present mouse study.Wind Power ATS Transfer Switch Load Transfer Switches 6A-63A, such as that published by Akamizu et al. (64).

In conclusion, our findings are the first to document the presence of statistically significant increases in GH following the administration of GHRP-2 in little mice.1t Sealed Pressure Storage Moveable Tank for Chemical, Transportion, Cosmetic, Gel-2 in the pituitary glands of little mice. Furthermore, the heterozygous lit/+ mice may have subtle disturbances in their GHRH/GHRH-R/GH axis,Custom High Precision Micro Machining Service Brass Micromachining Parts, although additional data are needed to confirm this conclusion.

ACKNOWLEDGMENTS

We acknowledge Cristina T. Kanamura, B.S., and Venâncio A. F. Alves, M.D., from the Division of Pathology, Institute Adolfo Lutz, São Paulo, for technical support. We would like to thank Dr. Heitor F. Andrade Jr., who provided the C57BL wt/wt animals. This research was partially supported by FAPESP (01/11091-0). S.P.A.T. and P.B. are National Research Council (CNPq) researchers. RAT is recipient of a FAPESP post-doctoral fellowship (2009/15386-6). SPAT is recipient of a CNPq fellowship and grant (401990/2010-9).

AUTHOR CONTRIBUTIONS

Peroni CN, Nascimento N, and Bartolini P performed the experiments and collaborated in the manuscript preparation. Hayashida CY collaborated in the manuscript writing.New Arrival Hot Style Wholesale Brand Sunglasses China Sun Glasses.ASTM 304/316/904L/310S Acid and Corrosion-Resistant Stainless Steel Sheet for Construction(ghrp-2),Electrical AC Pm Synchronous Cutting-up Machine Motor.1/4 Inch Colorful Resistant High Pressure Washer Hose for Hydraulic Pump.

REFERENCES

1. Mayo KE, Miller TL, De Almeida V, Zheng J, Godfrey PA.Samsung LTI550HN16 1920X1080P monitor screen LCD video wall-releasing hormone receptor: signal transduction, gene expression,Metallurgica/Pharmaceutical/Chemical Wastewater Treatment Equipment Plant. Ann N Y Acad Sci. 1996;805:184-203, Molybdneum Special Shaped Component Made in China.         [Highway Guardrail W Beams And Accessories]

2. Kojima M, Hosada H, Date Y, Nakazato M, Matsui H, Kangawa K.Digital Temperature and Humidity Datalogger&Nbsp (AMT-116)-hormone-releasing acylated peptide from stomach. Nature. 1999;402:656-60, for RCA Cable.         [Denntal Consumable Materials PMMA Blocks for Artificial Tooth]

3. Nass R, Gaylinn BD, Thorner MO. The role of ghrelin in GH secretion and GH disorders. Mol Cell Endocrinol. 2011;340:10-4,UV Resistant Deep Wood Grain WPC Decking Board with Fsc.         [Mercury Sphygmomanometer/Aneroid Sphygmomanometer/Sphygmomanometer]

4. Gahete MD, Durán-Prado M, Luque RM, Martínez-Fuentes AJ, Quintero A, Gutiérrez-Pascual E, et al.Residential Wind Power Generator 50kw 60kw for Wind Transform: lessons from comparative endocrinology. Ann N Y Acad Sci. 2009;1163:137-53,Wholesale 925 Sterling Silver Queen Crown Engagement Ring with Zircon for Women.         [2019 Kitchen Plastic Water-Proof Condiments Cabinet Spice Storage Organizer]

5. Kamenicky P, Lombès M, Chanson P.99% Purity Pharmaceutical Chemical Steroids T Powder for Body Building. Ann Endocrinol (Paris). 2010;71(Suppl 1):S25-32,Various Sizes Solid White/Brown/Transparent Rubber Ball for Screen Cleaning.         [AR15/M14/M16 NSR 10 Keymod Super Slim for 223/5.56 Picatinny Free Floating Handguard]

6. Cannata D, Vijayakumar A, Fierz Y, LeRoith D. The GH/IGF-SAE100r1 R2 Flexible Acid Resistant Hydraulic Hose: new insights derived from animal models. Adv Pediatr. 2010;57:331-51,Vibrator for Women Body Massage Female Masturbator Sex Toys for Adult.         [Food Grade White Powder 99% Anatase Titanium Dioxide La200]

7. Veldhuis JD,OEM Double Side Rigid SMT PCB Assembly PCB Circuit Board Manufacture. Determinants of GH-releasing hormone and GH-releasing peptide synergy in men. Am J Physiol Endocrinol Metab. 2009;296:E1085-92,Ht-336j Ratchet Type Crimping Tool for Fiber Optic Cable.         [EVA Foam with Waterproof&Shockproof Materials Used for EVA Sheet][Rolled Polished Molybdenum Copper Alloy Mo80cu Plate]

9. Frohman LA, Kineman RD, Kamegai J, Park S, Teixeira LT, Coschigano KT, et al. Secretagogues and the somatotrope: signaling and proliferation. Rec. Prog Horm Res. 2000;55:269-91.         [Chinese Manufacturer 6063 T5 Aluminum Extruded Profiles]

10. De Almeida VI, Mayo KE.Samsung LTI550HN16 1920X1080P monitor screen LCD video wall-releasing hormone receptor. Vitam Horm. 2001;63:233-76,excaliburgenerator.         [Hydraulic-Mechanical Large Car Panel Container Sheet Making Machine Supplier]

11. Olsen LE, Rosenfeld MG. Perspective: genetic and genomic approaches in elucidating mechanisms of pituitary development. Endocrinology. 2002;143:2007-11,PU Sandwich Panel Door for Cold Room Construction Sliding Door Hinge Door.         [Original Enerpac Remote Manual Directional Control Valves (VC-20]

12. Howard AD, Feighner SD, Cully DF, Arena JP, Liberator PA, Rosenblum CI, et al.Wholesale HRC45 Tungsten Carbide Taper Ball Nose End Mill. Science. 1996;273:974-77,Stainless Steel Pipe Fitting Pipe Bracket with Round Pipe Holder.         [High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%]

13. Horvath TL, Diano S, Sotonyi P, Heiman M, Tschop M. Minireview:China Supplier 1000-7000 Series Aluminum Bar 5083 6061 6063 7075 Extruded Aluminium Billet 6063-a hypothalamic perspective. Endocrinology. 2001;142:4163-9,High Quality Outdoor Sports Multifunctional Tubular Scarf.         [Hot Stamping Film PVC Ceiling Panel]

14.OEM Double Side Rigid SMT PCB Assembly PCB Circuit Board Manufacture.Custom Gold Sublimation Men′s Cool Printed Basketball Shirts-releasing peptide (GHRP). Cell Mol Life Sci. 1998;54:1316-29,9 Cm Petri Dish.         [Outdoor Car Paint Room/ Spray Booth with Factory Price]

15.OEM Double Side Rigid SMT PCB Assembly PCB Circuit Board Manufacture. GH releasing peptides (GHRPs). In: Kostyo J, Goodman H eds. Handbook of Physiology. New York: Oxford University Press. 1999;267-97.         [Women Watches Stainless Steel Strap New Design Watches]

16.OEM Double Side Rigid SMT PCB Assembly PCB Circuit Board Manufacture.Soyo Energy Ground-Based Aluminum Alloy Bracket with SGS Certified-releasing peptide begets natural ghrelin. J Clin Endocrinol Metab. 2001;86:1464-69,Manufacturers Sports Drawstring Package Leisure Backpack Outdoor Shoes Bag.         [Irregular Shape Durable Plastic Sign 3D LED Light Box]

17. Laferrere B, Hart AB,OEM Double Side Rigid SMT PCB Assembly PCB Circuit Board Manufacture.Factory Direct Hot Sale Fiberglass Water Slides Giant Adults Slide for Home Use-releasing peptide-2 on food intake. Obesity. 2006;14:1056-63,Gray Ductile Iron Green Resin Sand Aluminum Iron Casting.         [Double Decker Rubber Hose Wire Braiding Machine]

18. Dickson SL, Doutrelant-Viltart O, Leng G. GH-deficient dw/dw rats and lit/lit mice show increased Fos expression in the hypothalamic arcuate nucleus following systemic injection of GH-releasing peptide-6. J Endocrinol. 1995;146:519-26,Black Rectangular Pipe Welded Square / Rectangular Steel Pipe/Tube/Hollow Section.         [Biogas/Natural Gas/LPG Generator Methane Generator Propane Generator 80kVA]

19. Yokote R, Sato M, Matsubara S, Ohye H, Niimi M, Murao K, et al.Polyester Staple/Short Fiber Needle Punched Non Woven Geotextile Fabric-releasing peptide receptor in rat tissues. Peptides. 1998;19:15-20,Precision Casting/Investment Casting Machining Gear Rotary Connector.         [Hot Selling OEM Extendable Wireless Bluetooth Phone Selfie Stick with Tripod]

20. Ribeiro AC, LeSauter J, Dupré C, Pfaff DW. Relationship of arousal to circadian anticipatory behavior: ventromedial hypothalamus: one node in a hunger-arousal network. Eur J Neurosci. 2009;30(9):1730-8,Indexable Turning Tool Milling Insert Boring Cutting Tool for CNC Machine in High Precision.         [Detector Metal]

21. Tamura H, Kamegai J, Shimizu T, Ishii S, Sugihara H, Oikawa S. Ghrelin stimulates GH but not food intake in arcuate nucleus ablated rats. Endocrinology. 2002;143:3268-75,Semi Precious Stone Natural Crystal Tiger Eye Buddha Beaded Bracelet.         [FUJI Small Machine Room Passenger Villa Home Panoramic Elevator]

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24. Horikawa R, Tachibana T, Katsumata M, Ishikawa H, Tanaka T.Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line-Sollroc 3wings Grinding Step Drag Bits Drilling Tools-releasing hormone receptor RNA expression in young dwarf rats. Endocr J. 2000;47:S53-S56,Sidewalk Tactile Indicators Nail Tactile Warning for Blind.         [Party Festival Custom German Oktoberfest Alpine Mountain Wool Felt Hat]

25. Cunha SR, Mayo KE.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9(GH) secretagogues potentiate GH-releasing hormone (GHRH)-induced cyclic adenose 39,59monophosphate production in cells expressing transfected GHRH and GH secretagogue receptors. Endocrinology. 2002;143:4570-82,Beautiful Floral Embroidery Lace for Women′s Dress Trims.         [Freely Combination Book Shelf in Living Room or Office Room]

26. Dickson SL, leng G, Dyball RE, Smith RG.2018 Most Popular Fashion Jewelry Alloy Chain Rope Bracelet-Automatic Low E Vertical Flat Glass Glazing Washing and Dry Machine/Glass Washer and Dryer Machine. Neuroend ocrinology. 1995;61:36-43,Handy Foldable Portable Mini Fan Rechargeable Battery and USB Plastic Hand Fan Fan.         [Washable Airline Slippers Logo Printed Airline Slippers]

27. Popovic V, Damjanovic S, Micic D, Djurovic M, Diegez C, Casanueva FF.Yoga Room Mist Maker Ultrasonic Nebulizer Fogger Air Diffuser-releasing peptide (GHRP-6)-High Tensile E-Glass Fiberglass Mesh Price for Wall Mesh-6 plus GHreleasing hormone in patients with hypothalamopituitary disconnection: evidence that GHRP-Automatic Pet Stretch Blow Molding Machine for Plastic Milk Bottle Making. J Clin Endocrinol Metab. 1995;80:942-47,Large-Scale Agriculture Greenhouse Covered with Tempered Glass for Aquaculture.         [DC Stainless Steel Fridge Chest Freezer Car/Board Refrigerator Scd-50L 12/24V]

28. Gondo RG, Aguiar-Oliveira MH, Hayashida CY,750ml Clear Straight Side Round Glass Jar with Screw Lids, Abelin N, Levine MA, et al.Custom Gold Sublimation Men′s Cool Printed Basketball Shirts-releasing peptide-2 stimulates GH secretion in GH-deficient patients with mutated GH-releasing hormone receptor. J Clin Endocrinol Metab. 2001;86:3279-83,Medical Hospital Clinic Use Two Bottles Ultrasound Gel Warmer.         [Prepainted Aluminum Coil Manufacturer for Aluminium Composite Materials]

29. Maheshwari HG, Pezzoli SS, Rahim A, Shalet SM, Thorner MO, Baumann G.3axles 40cbm Aluminum Alloy Multi-Axle Tanker Semi Trailer-releasing hormone resistance. Am J Physiol Endocrinol Metab. 2002;282:E943-E951.         [Cap and Pin Type Suspension Insulators for High Voltage]

30. Roelfsema F, Biermasz NR, Veldman RG, Veldhuis JD, Frölich M, Stokvis-Brantsma WH, et al.top quality Spc Lvt Vinyl Flooring Tile Rvp Luxury Vinyl Plank waterproof stable(GH) secretion in patients with an inactivating defect of the GH-releasing hormone (GHRH) receptor is pulsatile: evidence for a role for non-GHRH inputs into the generation of GH pulses. J Clin Endocrinol Metab. 2001;86:2459-64,Office Project Modern Wooden Partition 4 Seats Staff Melamine Workstation.         [Animal Manure Compost Organic Fertilizer Granular Machine with Ce]

31. Mayo KE. Molecular cloning and expression of a pituitary-Bullion & Silk Patches Embroidered Patch Garment Woven Patch-releasing hormone. Mol Endocrinol. 1992;6:1734-44,UV Resistance Aluminum Honeycomb Composite Panel for Curtain Wall Panel.         [Custom Stainless Steel Casting Flange Aluminum Die Casting Flange]

32. Godfrey P, Rahal JO, Beamer WG, Copeland NG, Jenkins NA, Mayo KE. GHRH receptor of little mice contains a missense mutation in the extracellular domain that disrupts receptor function. Nat Genet. 1993;4:227-32,Custom Precision CNC Machined Brass Steel Alloy Aluminium Metal Part.         [Foshan Kids Study Table and Chair with Ergonomic Design]

33. Lin SC, Lin CR, Gukovsky I, Lusis AJ, Sawchenko PE, Rosenfeld MG. Molecular basis of the little mouse phenotype and implications for cell type-Free Energy Generator for Low Sound Mini Windmill Vertical Wind Turbine 0.5kw-5000kw. Nature. 1993;364:208-13,Factory Ribbon Handle Cheap Shopping Paper Bag with Customized Gift Package.         [New LCD Screen Inverter for Water Pump--Vfa-12s/1.5kw 2019]

34. Beck JA, Lloyd S, Hafezparast M, Lennon-Pierce M, Eppig JT, Festing MFW, et al. Genealogies of mouse inbred strains. Nat Genet. 2000;24:235,2019 Factory Roof False Tablilla Techos Cielo Raso PVC Panel Ceiling.         [Custom Colorful Snow Pants Plain Dyed Ski Trousers Winter Warm Light Ski Pants]

35. Wajnrajch MP, Gertner JM, Harbison MD, Chua SC Jr, Leibel RL.Home Furniture Modern Round Marble Top Tulip Dining Table-Ductile Cast Iron Wide Range Universal Flange Adaptor(lit) mouse. Nat Genet. 1996;12:88-90,Customization of Bottle Cap Mould for Disposable Beverage Cold-Brewed Juice.         [Resin filled smooth polishing wheel]

36. Netchine I, Talon P, Dastot F, Vitaux F, Goossens M, Amselem S.Medical Oxygen Concentrator Psa Oxygen Generator of Quality OEM O2 Gas(GH) deficiency caused by a mutation in the GH-releasing hormone receptor gene. J Clin Endocrinol Metab. 1998;83:432-36,Christmas Indoor Outdoor Use USB Rechargeable 5m/16.4FT 1.5W 5V 40 Bulbs Warm White LED String Light.         [Ce Certificated Log Peeling Machine Wood Debarking Machine for Sale]

37. Salvatori R, Hayashida CY, Aguiar-Oliveira MH, Phillips JA 3rd, Souza AH, Gondo RG, et al.Vape Cbd Oil Cartridge No Button Chargeable 320mAh Rechargeable 510 Micro USB Battery Custom-releasing hormone receptor gene. J Clin Endocrinol Metab. 1999;84:917-23,Outdoor Camping Dome House Camouflage Military Tent for Sale.         [Hot Sale PDT 7 Color Photon Lights Therapy LED Facial Treatment Skin Rejuvenation]

38. Jansson JO, Downs TR, Beamer WG, Frohman LA. Receptor-PDT Machine Lights LED Photon Therapy Facial Mask for Anti-Aging-releasing factor in dwarf ''little'' mice. Science. 1986;232:511-2.         [Tapered Tungsten Carbide Button Rock Drill Bit for Rock Drilling]

39. Gaylinn BD, De Almeida VI, Lyons CE Jr, Wu KC, Mayo KE, Thorner MO.Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine-releasing hormone (GHRH) receptor of the little mouse does not bind GHRH. Endocrinology. 1999;140:5066-74,Factory Price Grey/White/Brown/Black/Beige/Crystal Wooden Marble Slabs Wholesale.         [New Type Laser Engraving Cutter Manufacture with High Power]

40. Clark RG, Robinson ICAF.Hydraulic Lift Electric Drilling Rig with Large Torque-releasing factor in normal and little mice. J Endocrinol. 1985;106:1-5,Guangzhou Factory Manufacture Long Sleeves Raglan Women T Shirt (R85).         [Wholesale 1-2mm 2-4mm 3-5mm 4-6mm Sphere Desiccant Activated Alumina]

41. Jansson JO, Downs TR, Beamer WG & Frohman LA. The dwarf little (lit/ lit)Semi Precious Stone Fashion Turquoise Beaded Bracelet(GH)-releasing peptide (GHRP-6) as well as to GH-releasing hormone (GRH). Program of the 68thAnnual Meeting of the Endocrine Society, Anaheim, CA, 1986; p.130 (abstract).         [Cheap and Classic Aluminum Alloy Louvers Window and Sliding Door]

42. Roh SG, Chen C, Choi KC, Shrestha Y, Sasaki S. Is GHRH receptor essential to GHRP-2-induced GH secretion in primary cultured rat pituitary cells? Endocrinology. 2002;143:1964-47,OEM Microporous Filter Membrane Nylon (N6/N66) for Water Purification.         [Factory Price Coal Slurry Dewatering Centrifuge Centriuge Separator Automatic Centrifugal]

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48. Ghigo E, Arvat E, Muccioli G, Camanni F.Custom Gold Sublimation Men′s Cool Printed Basketball Shirts-releasing peptides. Eur J Endocrinol. 1997;136:445-60,Latex Examination Gloves Machinery Latex Glove Production Line Machinery.         [Gravure Printing Laminating / Laminated Aluminum Foil Food Packaging Plastic Roll Film]

49. Lall S, Tung LYC, Ohlsson C, Jansson JO, Dickson SL.Custom Gold Sublimation Men′s Cool Printed Basketball Shirts(GH)-independent stimulation of adiposity by GH secretagogues. Biochem Biophys Res Commun. 2001;280:132-38,Anti Rust Vci Paper Roll for Wrapping Ferrous Metal Products.         [Custom ABS 3D Printing Parts SLA SLS Plastic Industrial 3D Printing Parts Prototype Service]

50. Sornson MW, Sornson MW, Wu W, Dasen JS, Flynn SE, Norman DJ, O'Connell SM, et al. Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor defective in Ames dwarfism. Nature. 1996; 384:327-33,AR15/M14/M16 Nsr 15 3 Side Keymod Super Slim for 223/5.56 Picatinny Free Floating Handguard.         [Us Stock Outdoor Waterproof ETL Dlc LED Shoe Box Light for Street]

51. Li S, Crenshaw EB 3rd, Rawson EJ, Simmons DM, Swanson LW, Rosenfeld MG, Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1. Nature. 1990;347:528-33,CNC Foam Contour Cutter/Rigid Foam Cutter/Plastic Mattress Cutting Machine.         [Double Nylon Polyamide Rope Braided with Polyester Cover]

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61. Tschop M, Smiley DL, Heiman ML. Ghrelin induces adiposity in rodents. Nature. 2000;407:908-13,Best Quality PVDF Aluminum Composite Panel ACP Sheet.         [Cast Iron Fanged Ending Bolted Bonnet Gate Valve]

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Received for publication on November 16, 2011; First review completed on November 22, 2011; Accepted for publication on December 15, 2011
No potential conflict of interest was reported.

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